(r3) Barrick Lab :: ProtocolsRecipesSaline

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---+++ Sterile Saline 

*Purpose: Used make dilutions of viable cells for plating or transfer to new media.*

This saline concentration of 0.85% w/v (145 mM) is suitable for diluting _E. coli_ before transferring to new media or plating on agar.

|  *1L*  |  *5L*  |  *Component*  |
|  8.5 g  |  42.5 g  |  NaCl  | 

Add H<sub>2</sub>O to final volume. Autoclave before use. Prepare large batches in 6 L flask and then aliquot out into bottles. It is not important to measure out exact volumes when dividing a large batch among bottles.