(r3) Barrick Lab :: ProtocolsRecipesM9Minimal

---+++ M9 Minimal

Make 5&times; M9 salts:
| 64 g | Sodium phosphate (dibasic, 7 hydrate) Na<sub>2</sub>HPO<sub>4</sub>•H<sub>2</sub>O  | 
| 15 g | Potassium phosphate (monobasic) KH<sub>2</sub>PO<sub>4</sub> |
| 2.5 g | Sodium chloride, NaCl | 
| 5 g | Ammonium chloride NH<sub>4</sub>Cl | 
Add dH<sub>2</sub>O to 1 liter. Autoclave.

Combine the following ingredients (all pre-sterilized):
| 200 ml | 5&times; M9 salts  | 
| 2 ml | 1M Magnesium sulfate MgSO<sub>4</sub> |
| 0.1 ml | 1 M Calcium chloride CaCl<sub>2</sub> |
| 40 ml | 10% (w/v) glucose (or other carbon source) |
Add *sterile* dH<sub>2</sub>O to 1 liter. Autoclave. <br>

To make M9 plates add the items above to 16g of sterilized agar. <br>
If making less than 1 liter use 8g of agar per 500ml of solution. <br>

Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesM9Minimal

Contributors to this topic

CraigBarnhart, JordanMonk, JeffreyBarrick, AurkoDasgupta

Topic revision: r3 - 2011-06-27 - 19:38:33 - Main.CraigBarnhart