(r20) Barrick Lab :: ProtocolUpdatesNeeded

---+ Protocol Updates Needed (as of 2016_Dec_15)

---++ Protocols needed or flagged for updates
   * Flow protocol updates (Dan and Dacia)
   * P1 transduction; FLP/FRT recombination (Dan)
   * Chemical Competent Cells and Zcomp cells(Colin and Peng?)
   * ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility. (<- Dan?) 
   * ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells. 
   * ProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions
   * ProtocolsQuartzy - Instructions for using Quartzy to mark orders received, request supply orders, and order supplies for the lab
   * NewLabMemberChecklist - EHS training course list is out of date, computer setup for coding is incomplete/fewer people use MacPorts recently, also combine all online accounts under one header?
   * Update Gibson Assembly protocol - add images for clarity and future-proof language (location)
   
---++ Recently finished protocols (kept until next Hackathon)

   * ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid.  (finished!)
   * AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab (No longer relevant!)
   * ProtocolsncAAGFPIncorporationAssay - General instructions for a basic GFP Incorporation assay that can measure whether a particular synthetase pair incorporates a ncAA (we wrote  a paper about this....)
   * LabMemberContactInformation - Lab members who left years ago still on list, newer lab members don't have most info added (mostly up to date)

Barrick Lab > ProtocolList > ProtocolUpdatesNeeded

Contributors to this topic

JeffreyBarrick, SeanLeonard, CraigBarnhart, DanielDeatherage, AurkoDasgupta, JordanMonk, LindseyWolf, CameronRoots, SimonDAlton, SimonDOelsnitz, AlvaroRodriguez

Topic revision: r20 - 2016-12-15 - 20:37:08 - Main.SimonDOelsnitz