Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	4,615,673	IS150 (+) +4 bp	coding (145‑148/1233 nt)	nadR →	nicotinamide‑nucleotide adenylyltransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 3652533	NA (NA)	82 (1.270)	60/200	0.1	97.5%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	4615673 =	2 (0.030)					coding (145/1233 nt)	nadR	nicotinamide‑nucleotide adenylyltransferase
*	?	REL606	3893554 =	NA (NA)	66 (1.030)	52/200	0.2	96.9%	noncoding (1/1443 nt)	IS150	repeat region
	?	REL606	= 4615676	2 (0.030)					coding (148/1233 nt)	nadR	nicotinamide‑nucleotide adenylyltransferase

TGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3893650‑3893554 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTGGCATCAGCCACCTGCTGTAGC  <  REL606/4615676‑4615579                                                                                                                                                                                                     TGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTC                                                                                                >  2:105187/1‑101   ATTCACGACTTCAAGGTGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCA                                                                                              >  1:1157004/1‑101   ATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCA                                                                                              <  1:939391/101‑1   ATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCA                                                                                              >  1:1501193/1‑101   ATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCA                                                                                              >  1:1314846/1‑101     TCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGC                                                                                            >  2:1405098/1‑101        CGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTT                                                                                         >  1:1206043/1‑101         GACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTT                                                                                        >  2:1315152/1‑101         GACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTT                                                                                        <  2:1336340/101‑1          ACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTG                                                                                       <  1:545252/101‑1           CTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGC                                                                                      >  1:602170/1‑101             TCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGC                                                                                    >  2:1558016/1‑101             TCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGC                                                                                    <  2:21928/101‑1                   CGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGG                                                                              <  1:100166/101‑1                   CGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGG                                                                              >  2:1170054/1‑101                   CGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGG                                                                              <  2:1670953/101‑1                        TTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTT                                                                         <  1:789951/101‑1                        TTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTT                                                                         <  1:1315158/101‑1                         TCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTT                                                                        <  1:1269869/101‑1                          CAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTG                                                                       <  2:539319/101‑1                          CAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTG                                                                       <  1:1056402/101‑1                           AAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGA                                                                      >  1:1138549/1‑101                             AAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATT                                                                    <  1:1097537/101‑1                               GGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTT                                                                  <  2:1364975/101‑1                               GGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTT                                                                  <  1:1026665/101‑1                                 GTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGG                                                                >  2:1466211/1‑101                                     TTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATT                                                            <  2:939605/101‑1                                      TTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGTTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTC                                                           >  1:823442/1‑101                                      TTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTC                                                           <  2:600411/101‑1                                       TGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCA                                                          <  2:341151/101‑1                                       TGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCA                                                          <  2:1314840/101‑1                                        GGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAG                                                         <  1:1413300/101‑1                                        GGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAG                                                         <  2:357899/101‑1                                          CTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTA                                                       <  1:734978/101‑1                                           TTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGTTTTTGATTTTGGCATTCAGTAA                                                      >  2:234741/1‑101                                           TTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAA                                                      >  2:1255685/1‑101                                            ttaACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAAC                                                     <  1:1387887/98‑1                                             TGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACT                                                    >  1:274945/1‑101                                              GACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTG                                                   <  2:652311/101‑1                                                 ATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCT                                                >  2:1541785/1‑101                                                   ATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTA                                              >  1:551625/1‑101                                                   ATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTA                                              <  1:1681411/101‑1                                                      AGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAAT                                           <  1:1472318/101‑1                                                      AGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAAT                                           <  2:1301622/101‑1                                                            TATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTT                                     <  2:602165/101‑1                                                              TTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTG                                   >  1:492444/1‑101                                                              TTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTG                                   >  1:1210893/1‑101                                                              TTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTG                                   >  1:755444/1‑101                                                               TCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGG                                  <  1:1601456/101‑1                                                                  ATTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCA                               <  1:1558022/101‑1                                                                   TTTCATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCAT                              >  1:674030/1‑101                                                                      CATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACC                           <  2:904348/101‑1                                                                       ATCGTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCG                          >  1:919901/1‑101                                                                          GTCCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTG                       <  2:1020980/101‑1                                                                            CCAACAAAATGGGTGCAGTACCCCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTGGC                     <  2:596272/101‑1                                                                            CCAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTGGC                     >  1:968444/1‑101                                                                             CAACAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTGGCA                    >  1:1378309/1‑101                                                                                CAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTGGCATCA                 <  2:1176277/101‑1                                                                                CAAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTGGCATCA                 <  1:396954/101‑1                                                                                 AAAATGGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTGGCATCAG                >  1:1081845/1‑101                                                                                 AAAATGGGCGCAGTACACCTCCAGCTTTTTCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACTTCTGGCATCAG                >  2:292849/1‑101                                                                                      GGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTGGCATCAGCCACC           >  2:1014081/1‑101                                                                                      GGGTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGAAACAGGCTTAAATACCCTTTGGTCATACCGCTGGCATCAGCCACC           <  2:387465/101‑1                                                                                        GTGCAGTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTGGCATCAGCCACCTG         <  2:1535031/101‑1                                                                                             GTACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTGGCATCAGCCACCTGCTGTA    <  1:369181/101‑1                                                                                               ACACCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTGGCATCAGCCACCTGCTGTAGC  <  2:1035450/101‑1                                                                                                                                                                                                     TGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3893650‑3893554 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCTCCAGCTTTTGCGCGCTGGGGCTTTTGATTTTGGCATTCAGTAACTGGCTTAAATACCCTTTGGTCATACCGCTGGCATCAGCCACCTGCTGTAGC  <  REL606/4615676‑4615579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.