Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	4,415,710	IS150 (–) +3 bp	coding (848‑850/1413 nt)	cycA →	D‑alanine/D‑serine/glycine transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	3893554 =	NA (NA)	71 (1.100)	57/200	0.1	100%	noncoding (1/1443 nt)	IS150	repeat region
	?	REL606	4415710 =	0 (0.000)					coding (848/1413 nt)	cycA	D‑alanine/D‑serine/glycine transporter
*	?	REL606	= 3894996	NA (NA)	80 (1.260)	64/198	0.0	100%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	= 4415712	0 (0.000)					coding (850/1413 nt)	cycA	D‑alanine/D‑serine/glycine transporter

GTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3893651‑3893554 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTT  >  REL606/4415710‑4415809                                                                                                                                                                                                        GTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTG                                                                                                   >  2:430807/1‑101  TGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGT                                                                                                  >  1:1434889/1‑101  TGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGT                                                                                                  >  1:1547487/1‑101  TGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGT                                                                                                  <  2:1366569/101‑1         CGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTG                                                                                           <  1:1356860/101‑1           ACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTT                                                                                         <  1:456807/101‑1            CTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTC                                                                                        >  1:1509746/1‑101            CTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTC                                                                                        <  2:316748/101‑1             TTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCG                                                                                       >  1:323362/1‑101             TTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCG                                                                                       <  1:690860/101‑1                  GGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTG                                                                                  <  1:1363797/101‑1                  GGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTG                                                                                  <  2:854922/101‑1                  GGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTG                                                                                  <  1:19280/101‑1                   GCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGG                                                                                 >  1:795892/1‑101                   GCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGG                                                                                 <  1:430812/101‑1                    CGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGT                                                                                <  2:874718/101‑1                    CGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGT                                                                                >  1:1670091/1‑101                     GCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTA                                                                               <  2:1591372/101‑1                      CTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAG                                                                              >  2:1235924/1‑101                      CTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAG                                                                              <  2:1522117/101‑1                       TTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGG                                                                             >  2:1458065/1‑101                       TTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGG                                                                             <  1:731965/101‑1                       TTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGG                                                                             <  2:1623616/101‑1                           CAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTG                                                                         >  1:1256895/1‑101                              AAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCT                                                                      <  1:1136479/101‑1                              AAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCT                                                                      <  1:700315/101‑1                               AGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTG                                                                     >  1:157648/1‑101                                 GGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCT                                                                   >  1:158860/1‑101                                  GTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTG                                                                  <  2:1659608/101‑1                                    ATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCC                                                                <  1:1412346/101‑1                                    ATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCC                                                                <  2:554566/101‑1                                      TTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGC                                                              <  1:1185631/101‑1                                         GGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTATATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAG                                                           <  1:532015/101‑1                                          GCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGC                                                          <  1:469268/101‑1                                           CTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATGGTTGAACTGTGCGTGTTGCTAGGGCTGCCTGCTGCCGGGAGCG                                                         >  2:257192/1‑101                                             TTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTG                                                       >  1:868474/1‑101                                              TGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGA                                                      <  2:323362/101‑1                                                ACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATC                                                    >  2:417553/1‑101                                                 CATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCA                                                   >  1:900320/1‑101                                                 CATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCA                                                   >  1:461621/1‑101                                                  ATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAA                                                  >  2:1712249/1‑101                                                  ATATTAGGGGCTATTCCATTTCATCGTCCAACAAAAAGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGTGTGATCAA                                                  >  1:732662/1‑101                                                    ATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACT                                                >  1:343899/1‑101                                                       AGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTG                                             <  2:1626048/101‑1                                                        GGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGT                                            >  1:1577888/1‑101                                                         GGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTG                                           <  2:56758/101‑1                                                          GGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGG                                          >  2:520324/1‑101                                                           GCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGT                                         >  1:1598112/1‑101                                                            CTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTG                                        <  2:1425571/101‑1                                                                TCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGA                                    <  2:1701266/101‑1                                                                   ATTTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCT                                 >  1:854550/1‑101                                                                     TTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCT                               >  1:33136/1‑101                                                                     TTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCT                               >  2:1499788/1‑101                                                                     TTCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCT                               >  1:360110/1‑101                                                                      TCATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTG                              >  1:1243375/1‑101                                                                        ATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCG                            >  1:935807/1‑101                                                                        ATCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCG                            >  1:1553712/1‑101                                                                         cCGTCCAACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGG                           >  2:1156436/2‑101                                                                               AACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTT                     >  1:1286969/1‑101                                                                               AACAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTT                     >  2:524003/1‑101                                                                                 CAAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCC                   <  1:1156263/101‑1                                                                                  AAAATGGGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCG                  >  1:1459738/1‑101                                                                                        GGTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACA            >  2:22113/1‑101                                                                                         GTGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAG           >  2:108281/1‑101                                                                                          TGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGC          >  2:930285/1‑101                                                                                          TGCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGC          >  2:881105/1‑101                                                                                           GCAGTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCG         <  2:720959/101‑1                                                                                             atTACATTGTTGACCTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGCCCTCTGCGGCGTCTTCCGCTAACAGCGGC       <  2:37965/99‑1                                                                                              GTACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCG      <  2:174784/101‑1                                                                                                ACATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTC    <  2:157648/101‑1                                                                                                  ATTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTT  >  2:1714527/1‑101                                                                                                                                                                                                        GTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3893651‑3893554 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTGCTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTT  >  REL606/4415710‑4415809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.