Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	3,841,192	IS150 (+) +3 bp	coding (389‑391/1074 nt)	recF ←	recombination protein F

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	3841192 =	0 (0.000)	78 (1.210)	58/200	0.1	100%	coding (391/1074 nt)	recF	recombination protein F
	?	REL606	= 3894996	NA (NA)					noncoding (1443/1443 nt)	IS150	repeat region
*	?	REL606	= 3841194	0 (0.000)	95 (1.510)	66/196	0.0	100%	coding (389/1074 nt)	recF	recombination protein F
	?	REL606	3893554 =	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

ACGGTACAGACGGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3841290‑3841192 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTC  <  REL606/3894996‑3894900                                                                                                                                                                                                      ACGGTACAGACGGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTG                                                                                                 >  2:1319501/1‑101    GTACAGACGGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTAC                                                                                              >  1:956184/1‑101    GTACAGACGGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTAC                                                                                              <  1:661892/101‑1    GTACAGAAGGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTAC                                                                                              <  1:576137/101‑1     TACAGACGGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACT                                                                                             <  2:180680/101‑1       CAGACGGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGA                                                                                           >  2:365315/1‑101       CAGACGGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGA                                                                                           >  1:1465293/1‑101        AGACGGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGAC                                                                                          >  2:737355/1‑101          ACGGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCC                                                                                        <  2:262368/101‑1           CGGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCC                                                                                       <  2:610689/101‑1            GGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCA                                                                                      >  2:610520/1‑101              GCATAAGGTCGCGGACCTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAA                                                                                    >  2:1277047/1‑101               CATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAA                                                                                   >  1:540812/1‑101                ATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAA                                                                                  >  1:1071295/1‑101                ATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAA                                                                                  >  2:14995/1‑101                     GTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGG                                                                             >  2:225974/1‑101                       CGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGAC                                                                           <  2:174153/101‑1                       CGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGAC                                                                           <  1:1421362/101‑1                        GCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACA                                                                          <  1:424835/101‑1                         CGGAACTGGCGCACCTGATGCCAATGCAGTAGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAG                                                                         <  2:1541328/101‑1                            AACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTA                                                                      >  1:122796/1‑101                                 GCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACAC                                                                 <  2:444934/101‑1                                  CGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACG                                                                <  1:213716/101‑1                                   GCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGA                                                               >  2:174131/1‑101                                    CACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAG                                                              <  1:804306/101‑1                                    CACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAG                                                              <  1:1701390/101‑1                                     ACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGG                                                             >  2:1234973/1‑101                                     ACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGG                                                             >  2:738109/1‑101                                          ATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATAT                                                        >  2:829835/1‑101                                           TGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATA                                                       >  1:1172314/1‑101                                            GCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAG                                                      <  2:1661800/101‑1                                            GCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAG                                                      <  2:1439141/101‑1                                            GCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAG                                                      <  2:1437141/101‑1                                             CCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGG                                                     <  2:1087168/101‑1                                              CAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGT                                                    >  2:1562833/1‑101                                              CAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGT                                                    >  1:1068382/1‑101                                               AATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                                                   <  2:758668/101‑1                                                ATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCT                                                  >  1:915918/1‑101                                                    AGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATT                                              <  1:441999/101‑1                                                     GTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTC                                             >  1:1251553/1‑101                                                       TGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCG                                           >  2:748196/1‑101                                                         ATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGAT                                         <  2:1071290/101‑1                                                         ATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGAT                                         <  1:1047508/101‑1                                                         ATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGAT                                         <  1:1710169/101‑1                                                           AACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATAT                                       <  2:1054911/101‑1                                                                CAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAAT                                  >  1:1337204/1‑101                                                                CAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAAT                                  <  2:1633319/101‑1                                                                CAGAAGGGTTTACTTTACTCAACAGAGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACCGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAAT                                  >  2:1287601/1‑101                                                                 AGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATT                                 <  1:121554/101‑1                                                                 AGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATT                                 >  1:1276634/1‑101                                                                 AGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATT                                 <  1:233435/101‑1                                                                  GAAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTG                                >  2:127455/1‑101                                                                   AAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGG                               <  1:895698/101‑1                                                                   AAGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGG                               <  1:1185206/101‑1                                                                    AGGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGA                              >  1:119886/1‑101                                                                     GGGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAG                             <  2:122796/101‑1                                                                      GGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGT                            >  2:791099/1‑101                                                                      GGTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGT                            >  2:335402/1‑101                                                                       GTTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC                           >  1:872539/1‑101                                                                        TTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA                          >  2:1695539/1‑101                                                                        TTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA                          >  2:1143846/1‑101                                                                        TTTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA                          >  1:554556/1‑101                                                                         TTACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAG                         <  1:306600/101‑1                                                                          TACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA                        >  1:1465919/1‑101                                                                          TACTTTACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA                        >  1:65380/1‑101                                                                              TTACTAAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTGTGATTCCGATATTCAATTGGAGTCAGACCTT                    >  1:1225083/1‑101                                                                               TACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTT                   <  1:920672/101‑1                                                                                ACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTT                  <  1:1487317/101‑1                                                                                ACTCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTT                  >  1:790691/1‑101                                                                                  TCAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAA                <  1:1078455/101‑1                                                                                   CAACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAAT               <  1:179793/101‑1                                                                                    AACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATT              <  1:1495137/101‑1                                                                                    AACGGCGGCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATT              >  2:376903/1‑101                                                                                           GCCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCT       <  2:445853/101‑1                                                                                            CCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTA      <  2:1625841/101‑1                                                                                            CCCCAAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTA      <  1:1212296/101‑1                                                                                                AAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTC  >  2:294386/1‑101                                                                                                AAATTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTC  >  1:141847/1‑101                                                                                                                                                                                                      ACGGTACAGACGGGCATAAGGTCGCGGAACTGGCGCACCTGATGCCAATGCAGTTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3841290‑3841192 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTC  <  REL606/3894996‑3894900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.