Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	3,537,178	+GTACTGACCCCAAAA :: IS150 (–) :: +CAA	coding (40/2199 nt)	zntA →	zinc, cobalt and lead efflux system

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 666133	NA (NA)	89 (1.570) +TGGGGTCAGTAC	66/176	0.0	99.0%	pseudogene (355/387 nt)	gltL	ATP‑binding protein of glutamate/aspartate transport system; b0652_1
	?	REL606	= 3537178	1 (0.020)					coding (40/2199 nt)	zntA	zinc, cobalt and lead efflux system
*	?	REL606	3537179 =	1 (0.020)	67 (1.070) +TTG	52/194	0.2	98.6%	coding (41/2199 nt)	zntA	zinc, cobalt and lead efflux system
	?	REL606	3651091 =	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

CGGAGACGTTTTCAGAGAGCGTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3537277‑3537179 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC  >  REL606/3651091‑3651188                                                                                                    |||                                                                                                   CGGAGACGTTTTCAGAGAGCGTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATT                                                                                                     <  2:673567/101‑1 CGGAGACGTTTTCAGAGAGCGTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATT                                                                                                     <  1:33224/101‑1  GGAGACGTTTTCAGAGAGCGTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTG                                                                                                    >  1:1356118/1‑101   GAGACGTTTTCAGAGAGCGTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGT                                                                                                   >  2:1017984/1‑101       CGTTTTCAGAGAGCGTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTAC                                                                                               <  1:93998/101‑1       CGTTTTCAGAGAGCGTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTAC                                                                                               >  2:1497352/1‑101             CAGAGAGCGTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACC                                                                                         >  1:616398/1‑101                AGAGCGTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCAT                                                                                      >  1:1671434/1‑101                 GAGCGTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATT                                                                                     <  2:635087/101‑1                 GAGCGTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATT                                                                                     >  2:1653908/1‑101                     GTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGT                                                                                 >  2:1137791/1‑101                       TGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTG                                                                               <  2:848770/101‑1                       TGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTG                                                                               >  1:404946/1‑101                          CGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGAC                                                                            >  2:205644/1‑101                              CTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATG                                                                        >  1:810577/1‑101                              CTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATG                                                                        <  1:1014591/101‑1                               TGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGA                                                                       >  2:358569/1‑101                                GGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAA                                                                      <  2:478265/101‑1                                GGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAA                                                                      <  1:1064686/101‑1                                  AACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAAT                                                                    <  1:1284612/101‑1                                  AACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAAT                                                                    <  1:1653914/101‑1                                    CATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGG                                                                  <  1:158/101‑1                                       GCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAT                                                               <  1:1329183/101‑1                                       GCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAT                                                               <  2:1295774/101‑1                                        CGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATA                                                              <  1:207094/101‑1                                         GCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAG                                                             <  1:322570/101‑1                                         GCCGTCGCAGCAACAGTCGTTGGCGATCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTCGACGGTGAAATGGAATAG                                                             >  2:80493/1‑101                                            GTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCC                                                          >  2:1226785/1‑101                                              CGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCT                                                        >  2:870178/1‑101                                               GCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTA                                                       >  2:7654/1‑101                                                 AGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                     <  1:317799/101‑1                                                 AGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                     >  2:766823/1‑101                                                 AGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                     >  2:765617/1‑101                                                 AGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                     >  1:152782/1‑101                                                  GCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATA                                                    >  1:521504/1‑101                                                   CAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                   <  1:623025/101‑1                                                   CAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                   <  2:521499/101‑1                                                       AGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCA                                               <  2:754436/101‑1                                                        GTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAA                                              <  2:1245928/101‑1                                                          CGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG                                            >  2:222742/1‑101                                                          CGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG                                            <  2:879969/101‑1                                                                CGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAA                                      >  1:959718/1‑101                                                                CGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAA                                      <  1:1137796/101‑1                                                                 GTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAT                                     <  1:682566/101‑1                                                                 GTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAT                                     >  2:682561/1‑101                                                                 GTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAT                                     >  1:1627710/1‑101                                                                     TGTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCC                                 <  2:898157/101‑1                                                                      GTACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCT                                <  2:16503/101‑1                                                                        ACCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTT                              <  2:1388262/101‑1                                                                         CCGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTT                             <  2:730441/101‑1                                                                          CGTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACCATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATGCCCTTTTG                            <  1:1363913/101‑1                                                                           GTGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGA                           <  1:206457/101‑1                                                                            TGGTTAGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA                          <  1:561926/101‑1                                                                                 AGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCG                     <  1:1557050/101‑1                                                                                 AGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCG                     <  2:152782/101‑1                                                                                 AGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCG                     <  2:285614/101‑1                                                                                 AGCGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCG                     <  2:157847/101‑1                                                                                   CGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCC                   <  1:311642/101‑1                                                                                   CGGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCC                   <  1:929466/101‑1                                                                                    GGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCT                  >  1:685157/1‑101                                                                                    GGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCT                  >  1:855966/1‑101                                                                                    GGTTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCT                  >  1:1144648/1‑101                                                                                      TTTGAACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTG                <  1:302244/101‑1                                                                                          AACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGT            <  1:980061/101‑1                                                                                          AACGCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGT            >  1:1289376/1‑101                                                                                             GCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGT         >  2:1604804/1‑101                                                                                             GCAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGT         >  2:1615791/1‑101                                                                                              CAGCAATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTG        >  1:1322384/1‑101                                                                                                   ATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCA   >  1:1217574/1‑101                                                                                                   ATTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCA   >  2:1183904/1‑101                                                                                                    TTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC  <  1:1497358/101‑1                                                                                                    TTGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC  <  2:141010/101‑1                                                                                                    |||                                                                                                   CGGAGACGTTTTCAGAGAGCGTTGGCGAGCTGGAACATGCGCCGTCGCAGCAACAGTCGTTGGCGTTCTGTACCGTGGTTAGCGGTTTGAACGCAGCAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3537277‑3537179 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC  >  REL606/3651091‑3651188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.