Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	1,448,695	IS1 (+) +9 bp	coding (224‑232/1758 nt)	ynbC →	predicted hydrolase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	1448695 =	0 (0.000)	43 (0.670)	40/200	0.7	100%	coding (224/1758 nt)	ynbC	predicted hydrolase
	?	REL606	= 1460927	NA (NA)					noncoding (768/768 nt)	IS1	repeat region
*	?	REL606	= 1448703	0 (0.000)	50 (0.780)	37/198	0.9	100%	coding (232/1758 nt)	ynbC	predicted hydrolase
	?	REL606	1460160 =	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

TCCAGTCCAACCTGGCTGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/1448791‑1448695 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTT  <  REL606/1460927‑1460832                                                                                                                                                                                                   TCCAGTCCAACCTGGCTGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTA                                                                                              <  2:1333259/101‑1    AGTCCAACCTGGCTGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATG                                                                                           <  2:420576/101‑1    AGTCCAACCTGGCTGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATG                                                                                           >  1:1282342/1‑101     GTCCAACCTGGCTGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGA                                                                                          <  1:678809/101‑1         AACCTGGCTGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCC                                                                                      <  2:1493382/101‑1         AACCTGGCTGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCC                                                                                      >  1:984347/1‑101          ACCTGGCTGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCA                                                                                     <  2:1452441/101‑1            CTGGCTGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAAC                                                                                   <  2:1680190/101‑1             TGGCTGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACT                                                                                  >  1:544107/1‑101                CTGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTAT                                                                               >  2:1591406/1‑101                 TGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATT                                                                              >  1:1039394/1‑101                  GTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTG                                                                             >  1:135014/1‑101                    CGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGAT                                                                           <  1:586315/101‑1                    CGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGAT                                                                           <  2:1066120/101‑1                    CGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGAT                                                                           <  1:1339218/101‑1                    CGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGAT                                                                           <  2:1490500/101‑1                           AGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTT                                                                    <  2:833621/101‑1                            GCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTT                                                                   <  2:1556919/101‑1                             CAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTA                                                                  <  2:1337866/101‑1                              AAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTAT                                                                 <  2:195817/101‑1                                 ACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTT                                                              <  2:962919/101‑1                                  CGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTC                                                             <  2:946613/101‑1                                       AAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATA                                                        >  1:162131/1‑101                                            CATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCC                                                   <  2:665056/101‑1                                             ATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCC                                                  >  2:122968/1‑101                                               CGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGA                                                >  1:1069942/1‑101                                                 ACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATG                                              <  1:761898/101‑1                                                  CATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGA                                             <  1:239539/101‑1                                                    TCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACT                                           <  2:784885/101‑1                                                     CCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTT                                          >  1:905773/1‑101                                                          ACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCA                                     >  1:1277247/1‑101                                                           CTGAACGCGCAAGCGATGGGCTATAACCACGTGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCAT                                    <  2:598813/101‑1                                                                   cgAAGAGATCGGCTATAGCCACGCGGCCCCGCGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCC                            <  2:1719032/99‑1                                                                     AAGAGATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCAC                          >  1:82005/1‑101                                                                          ATGGGCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATT                     >  1:1006381/1‑101                                                                              GCTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGA                 <  2:1069937/101‑1                                                                               CTATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAG                >  2:1307263/1‑101                                                                                TATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGA               <  2:139301/101‑1                                                                                 ATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAA              >  2:1550758/1‑101                                                                                 ATAACCACGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAA              <  1:211888/101‑1                                                                                        CGCGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGC       <  1:452240/101‑1                                                                                          CGGCCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGA     >  2:1483854/1‑101                                                                                             CCCCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTT  <  2:1282337/101‑1                                                                                                                                                                                                   TCCAGTCCAACCTGGCTGTCGCTGGCAGCAAAACGGACAAATTCATCGACATCCCGCACTGAACGCGCAAGAGATGGGCTATAACCACGCGGCCCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/1448791‑1448695 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTT  <  REL606/1460927‑1460832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.