Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	1,448,695	IS1 (+) +9 bp	coding (224‑232/1758 nt)	ynbC →	predicted hydrolase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	1448695 =	0 (0.000)	43 (0.670)	40/200	0.7	100%	coding (224/1758 nt)	ynbC	predicted hydrolase
	?	REL606	= 1460927	NA (NA)					noncoding (768/768 nt)	IS1	repeat region
*	?	REL606	= 1448703	0 (0.000)	50 (0.780)	37/198	0.9	100%	coding (232/1758 nt)	ynbC	predicted hydrolase
	?	REL606	1460160 =	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

CATTCTGGTCGTCTACAACATCTCGTTGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/1448604‑1448703 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAC  >  REL606/1460160‑1460259                                                                                                                                                                                                          CATTCTGGTCGTCTACAACATCTCGTTGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCG                                                                                                     <  2:1426603/101‑1   TTCTGGTCGTCTACAACATCTCGTTGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGT                                                                                                   <  2:1078616/101‑1      TGGTCGTCTACAACATCTCGTTGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGAT                                                                                                >  1:1381709/1‑101            TCTACAACATCTCGTTGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCC                                                                                          <  2:217101/101‑1            TCTACAACATCTCGTTGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCC                                                                                          <  1:88304/101‑1             CTACAACATCTCGTTGATGAACTTGCGATGCCAGATACTGATTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTGCGGGGCGGCGGTGATGCTGCCA                                                                                         >  2:154678/1‑101              TACAACATCTCGTTGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAA                                                                                        >  1:95477/1‑101                CAACATCTCGTTGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACT                                                                                      <  2:959215/101‑1                  ACATCTCGTTGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTA                                                                                    <  2:1193915/101‑1                   CATCTCGTTGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTAC                                                                                   >  2:1497409/1‑101                         GTTGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTT                                                                             >  2:327958/1‑101                           TGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAG                                                                           <  2:924077/101‑1                            GATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGT                                                                          <  2:1200120/101‑1                                 ACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATG                                                                     <  2:733845/101‑1                                   TGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGAT                                                                   >  2:596121/1‑101                                    GGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATG                                                                  <  2:1381703/101‑1                                       GATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTG                                                               >  2:1291205/1‑101                                        ATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGT                                                              <  1:318629/101‑1                                          GCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTT                                                            <  2:147225/101‑1                                          GCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTT                                                            >  1:770180/1‑101                                              GATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGA                                                        >  2:629724/1‑101                                                 ACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGT                                                     >  1:1682526/1‑101                                                   TGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGCGTTTTTGAGGGGC                                                   >  2:570053/1‑101                                                           ATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGG                                           >  1:1407625/1‑101                                                             GCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCACGGTGATGCTGCCATCTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCT                                         <  2:1425557/101‑1                                                              CCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTT                                        >  2:1377056/1‑101                                                                   GATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTT                                   >  1:711675/1‑101                                                                   GATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTT                                   <  2:1604036/101‑1                                                                     TGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTC                                 <  2:722630/101‑1                                                                       gCCGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTA                               <  1:1102556/100‑1                                                                         CGAGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATC                             <  2:1251015/101‑1                                                                           AGGGCATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAG                           >  2:318237/1‑101                                                                                ATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTTTTAGCTGTC                      >  2:1538348/1‑101                                                                                ATGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTC                      >  1:1056744/1‑101                                                                                 TGGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCC                     >  1:879869/1‑101                                                                                  GGAAAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCC                    <  1:629729/101‑1                                                                                     AAAAGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCC                 >  2:894010/1‑101                                                                                        AGTTCGGGGCCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGT              >  1:799180/1‑101                                                                                                 CCGCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACT     <  2:890635/101‑1                                                                                                  CGCGTTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTG    <  2:328614/101‑1                                                                                                   GCGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGA   <  2:494823/101‑1                                                                                                    CGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAC  <  2:601997/101‑1                                                                                                    CGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAC  <  1:1609203/101‑1                                                                                                    CGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAC  <  1:944703/101‑1                                                                                                    CGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAC  >  1:1378338/1‑101                                                                                                    CGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAC  <  2:734290/101‑1                                                                                                    CGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAC  >  2:597965/1‑101                                                                                                    CGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAC  <  1:746641/101‑1                                                                                                    CGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAC  >  1:946934/1‑101                                                                                                    CGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAC  <  2:956322/101‑1                                                                                                    CGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAC  <  2:1464817/101‑1                                                                                                                                                                                                          CATTCTGGTCGTCTACAACATCTCGTTGATGAACTGGCGATGCCAGATACTGCTTTTTATGCCTGGGATGCCCGAGGGCATGGAAAAAGTTCGGGGCCGCg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/1448604‑1448703 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGAC  >  REL606/1460160‑1460259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.