Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	1,270,660	IS150 (–) +4 bp	intergenic (‑167/+235)	ldrC ← / ← chaA	toxic polypeptide, small/calcium/sodium:proton antiporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	664688 =	NA (NA)	59 (0.940)	52/196	0.2	96.6%	noncoding (1/1443 nt)	IS150	repeat region
	?	REL606	1270660 =	2 (0.030)					intergenic (‑167/+238)	ldrC/chaA	toxic polypeptide, small/calcium/sodium:proton antiporter
*	?	REL606	= 666130	NA (NA)	77 (1.200)	56/200	0.1	97.4%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	= 1270663	2 (0.030)					intergenic (‑170/+235)	ldrC/chaA	toxic polypeptide, small/calcium/sodium:proton antiporter

GATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/664783‑664688 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caCCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAG  >  REL606/1270660‑1270758                                                                                                                                                                                                     GATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGA                                                                                                <  2:370444/101‑1  ATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGAT                                                                                               >  2:129641/1‑101    TCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAA                                                                                             <  1:1072670/101‑1     CACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAAT                                                                                            <  2:1071484/101‑1     CACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAAT                                                                                            <  2:1684004/101‑1      attACTTCAAGGCGCTTTTCCAAAGCGTATTTTGGCTTTGACATATTAGGGGCTATTCCAGTTCATCGTCCAACAAAATGGGTGCACTTAACCGGATAATT                                                                                           <  2:1617712/98‑1        GACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCC                                                                                         >  1:1548066/1‑101          CTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGG                                                                                       >  2:991182/1‑101            TCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCT                                                                                     <  2:93145/101‑1             CAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTT                                                                                    <  2:696995/101‑1                  CGCTTTTCAACAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACCCCGGATAATTCCGGCTTGGTGT                                                                               <  2:779765/101‑1                   GCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTG                                                                              >  1:1316919/1‑101                      TTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGAT                                                                           >  2:607062/1‑101                       TTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATA                                                                          <  2:78175/101‑1                        TCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATAC                                                                         <  1:505640/101‑1                        TCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATAC                                                                         <  2:1555675/101‑1                         CAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACT                                                                        <  2:214029/101‑1                         CAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACT                                                                        >  2:1389282/1‑101                           AAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTAC                                                                      >  1:1387307/1‑101                            AAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACT                                                                     <  1:263909/101‑1                            AAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACT                                                                     >  2:255707/1‑101                                  ATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAA                                                               >  2:486491/1‑101                                   TTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAAT                                                              >  2:1291880/1‑101                                       GGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCAT                                                          >  2:120237/1‑101                                         CTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCT                                                        <  1:1157825/101‑1                                           TTGACATATTAGGGGCTATGCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTC                                                      <  2:803012/101‑1                                             GACATATTAGGGGCTATTCCATTTCATCATCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCAC                                                    >  2:111877/1‑101                                               CATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTT                                                  >  2:316459/1‑101                                                  ATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCAT                                               >  2:1108025/1‑101                                                    TAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCC                                             <  1:1520160/101‑1                                                     AGGGGCTATTCCATTTCATCTTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTAATTCTCAATTCATCTTCAGCTCATTCC                                            >  2:1233213/1‑101                                                     AGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCAGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCC                                            <  1:1045187/101‑1                                                         GCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAA                                        >  1:1357856/1‑101                                                         GCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAA                                        >  1:310854/1‑101                                                           TATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAAT                                      >  1:942829/1‑101                                                           TATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAAT                                      >  1:1309348/1‑101                                                            ATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATG                                     <  1:944906/101‑1                                                             TTCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGT                                    >  2:721352/1‑101                                                              TCCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTT                                   <  2:1366355/101‑1                                                              TCCATTTCATCGTCCAACAAAATGGGGGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAACTGTT                                   >  2:1653704/1‑101                                                               CCATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTT                                  >  2:485728/1‑101                                                                CATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTG                                 >  2:358611/1‑101                                                                CATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTG                                 <  1:1381693/101‑1                                                                 ATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGa                                >  1:1182203/1‑100                                                                 ATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGC                                <  2:706786/101‑1                                                                 ATTTCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTAGC                                <  1:1429272/101‑1                                                                    TCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAAT                             <  2:504409/101‑1                                                                    TCATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAAT                             <  2:1364897/101‑1                                                                      ATCGTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAA                           <  1:1663002/101‑1                                                                         GTCCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGA                        <  2:1316913/101‑1                                                                           CCAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGT                      <  1:32183/101‑1                                                                            CAACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTA                     >  2:515284/1‑101                                                                             AACAAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTAC                    <  2:942824/101‑1                                                                                AAAGTGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATT                 >  1:186755/1‑101                                                                                AAAATGGGTGCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATT                 <  2:1223098/101‑1                                                                                         GCAGTACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTT        <  1:1571116/101‑1                                                                                             TACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAAC    >  1:415217/1‑101                                                                                              ACACCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACA   >  2:669138/1‑101                                                                                              ACACCGGATAATTCCGGCTTGGGGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACA   >  2:1188236/1‑101                                                                                               CACCGGATAATTCCGGCTTGGGGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAG  >  1:1652362/1‑101                                                                                                                                                                                                     GATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/664783‑664688 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caCCGGATAATTCCGGCTTGGTGTGGATACTACTTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAG  >  REL606/1270660‑1270758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.