Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	336,055	IS150 (–) +3 bp	coding (2805‑2807/3075 nt)	lacZ ←	beta‑D‑galactosidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	336055 =	2 (0.030)	65 (1.010)	57/200	0.1	96.9%	coding (2807/3075 nt)	lacZ	beta‑D‑galactosidase
	?	REL606	588495 =	NA (NA)					noncoding (1/1977 nt)	IS150	repeat region
*	?	REL606	= 336057	2 (0.030)	77 (1.210)	57/198	0.1	97.4%	coding (2805/3075 nt)	lacZ	beta‑D‑galactosidase
	?	REL606	= 590471	NA (NA)					noncoding (1977/1977 nt)	IS150	repeat region

CAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/335958‑336057 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTC  <  REL606/590471‑590375                                                                                                                                                                                                       CAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTT                                                                                                  >  1:996059/1‑101  AGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTG                                                                                                 >  2:1352242/1‑101   GTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGT                                                                                                >  2:524122/1‑101   GTTGCTGTTGACTGGAGCGGCTGGTGTTGAACTGGAAGCCCCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGT                                                                                                >  2:743990/1‑101    TTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTA                                                                                               <  2:1094330/101‑1       CTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTG                                                                                            >  2:782424/1‑101        TGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGA                                                                                           >  2:1104558/1‑101         GTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGAC                                                                                          >  2:833020/1‑101              CTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAA                                                                                     >  2:646630/1‑101               TGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAA                                                                                    >  1:36198/1‑101                  AGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAG                                                                                 <  2:995289/101‑1                  AGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAG                                                                                 <  1:163809/101‑1                     GGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTG                                                                              <  2:143911/101‑1                      GCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGG                                                                             <  1:1352248/101‑1                        TGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGAC                                                                           >  2:272453/1‑101                         GATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACA                                                                          <  1:1266820/101‑1                          ATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAG                                                                         <  1:683094/101‑1                          ATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAG                                                                         <  2:1159173/101‑1                          ATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAG                                                                         >  1:44809/1‑101                          ATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAG                                                                         <  2:89363/101‑1                          ATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAG                                                                         <  1:1391042/101‑1                           TGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGT                                                                        <  1:524127/101‑1                           TGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGT                                                                        <  2:469548/101‑1                              TGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAA                                                                     >  2:1213519/1‑101                               GAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAA                                                                    >  1:720625/1‑101                                AACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAAC                                                                   <  2:1530753/101‑1                                  CTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGGCCCCAAAAAGTTGGACAGTTAAACAC                                                                 <  2:1103025/101‑1                                  CTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACAC                                                                 <  1:959530/101‑1                                   TGGAAGTCGCCGCGCCACTGGTGTGGGCTATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACt                                                                <  1:1647902/101‑2                                     GAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTATTGACCCCAAAAAGTTGGACAGTTAAACACGAG                                                              <  1:130019/101‑1                                       AGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGC                                                            >  1:1331697/1‑101                                         TCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCAT                                                          <  2:44809/101‑1                                          CGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATA                                                         >  2:572098/1‑101                                             CGCGCCACTGGTGTGGGTTATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTGTTGTACTGGCCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAG                                                      <  2:1549597/101‑1                                             CGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAG                                                      >  1:508658/1‑101                                                GCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCTAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                                                   >  1:198538/1‑101                                                GCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                                                   >  1:1345578/1‑101                                                GCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                                                   >  1:244498/1‑101                                                  CACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTG                                                 >  1:291006/1‑101                                                      GGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTC                                             <  1:743995/101‑1                                                       GTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCC                                            <  1:950133/101‑1                                                       GTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCC                                            <  1:1373032/101‑1                                                          TGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGAT                                         <  1:312373/101‑1                                                          TGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGAT                                         >  1:426572/1‑101                                                           GGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATA                                        >  1:1581797/1‑101                                                               CATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCA                                    >  2:679658/1‑101                                                               CATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCA                                    >  2:827510/1‑101                                                                 TAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAAT                                  >  1:849792/1‑101                                                                 TAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAAT                                  >  2:84121/1‑101                                                                 TAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAAT                                  <  1:782429/101‑1                                                                 TAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAAT                                  <  2:1561565/101‑1                                                                  AATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAATTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATT                                 <  2:544369/101‑1                                                                   ATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTG                                >  2:485568/1‑101                                                                    TTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGG                               <  2:447575/101‑1                                                                    TTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGG                               <  2:1610523/101‑1                                                                        ATTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC                           <  1:635091/101‑1                                                                         TTCGCGCGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA                          <  2:256830/101‑1                                                                               CGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTT                    <  1:309537/101‑1                                                                               CGTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTT                    >  1:519299/1‑101                                                                                GTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTT                   >  1:943450/1‑101                                                                                GTCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTT                   >  1:1354710/1‑101                                                                                 TCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTT                  <  2:1161420/101‑1                                                                                 TCCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTT                  >  2:54680/1‑101                                                                                  CCCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTA                 <  1:1174046/101‑1                                                                                   CCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAA                <  1:272453/101‑1                                                                                   CCGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAA                <  2:1043033/101‑1                                                                                    CGCAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAAT               <  1:93225/101‑1                                                                                    CGCAGCGCAAACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAAT               <  1:579435/101‑1                                                                                      CAGCGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTT             >  2:363335/1‑101                                                                                         CGCAGACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAG          <  1:617347/101‑1                                                                                             GACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTA      <  2:776220/101‑1                                                                                              ACCGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAA     >  1:14055/1‑101                                                                                                CGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATT   >  2:943558/1‑101                                                                                                CGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATT   <  1:1353166/101‑1                                                                                                CGTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATT   >  1:254758/1‑101                                                                                                 GTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTC  >  1:1630080/1‑101                                                                                                 GTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTC  >  2:220493/1‑101                                                                                                 GTTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTC  <  1:1397420/101‑1                                                                                                                                                                                                       CAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/335958‑336057 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTC  <  REL606/590471‑590375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.