Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	336,055	IS150 (–) +3 bp	coding (2805‑2807/3075 nt)	lacZ ←	beta‑D‑galactosidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	336055 =	2 (0.030)	65 (1.010)	57/200	0.1	96.9%	coding (2807/3075 nt)	lacZ	beta‑D‑galactosidase
	?	REL606	588495 =	NA (NA)					noncoding (1/1977 nt)	IS150	repeat region
*	?	REL606	= 336057	2 (0.030)	77 (1.210)	57/198	0.1	97.4%	coding (2805/3075 nt)	lacZ	beta‑D‑galactosidase
	?	REL606	= 590471	NA (NA)					noncoding (1977/1977 nt)	IS150	repeat region

GCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/336153‑336055 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGA  >  REL606/588495‑588587                                                                                                                                                                                                  GCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATG                                                                                             >  2:18774/1‑101  CAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGT                                                                                            <  2:1212339/101‑1    AGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTAC                                                                                          >  2:1152946/1‑101      AAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTG                                                                                        <  2:1159056/101‑1       AAACTATCCCGCCCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGC                                                                                       <  2:101737/101‑1        AACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCA                                                                                      >  2:1624768/1‑101         ACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCAC                                                                                     <  1:478521/101‑1         ACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCAC                                                                                     >  1:597086/1‑101          CTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACC                                                                                    <  1:1384627/101‑1           TATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCC                                                                                   <  1:737285/101‑1           TATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCC                                                                                   <  1:819916/101‑1             TCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCAT                                                                                 <  2:692527/101‑1             TCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCAT                                                                                 <  2:1226632/101‑1              CCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATT                                                                                <  1:978483/101‑1                CGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTT                                                                              >  2:939986/1‑101                 GACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTG                                                                             >  2:769980/1‑101                   CCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTT                                                                           >  2:739834/1‑101                   CCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTT                                                                           <  1:1486475/101‑1                     GCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGG                                                                         <  1:1624774/101‑1                          ACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGAT                                                                    >  1:1626954/1‑101                            TGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGA                                                                  >  2:1405212/1‑101                              CCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAA                                                                <  2:1305876/101‑1                               CGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAAT                                                               >  2:403387/1‑101                                 CCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGAAGATGAAATGG                                                             >  2:1482869/1‑101                                  CTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGA                                                            <  1:851477/101‑1                                   TGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAA                                                           >  2:1443648/1‑101                                    GTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAAT                                                          >  1:1617503/1‑101                                     TTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATA                                                         <  1:705704/101‑1                                        TGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCC                                                      <  2:279720/101‑1                                        TGACCGCTGCGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCC                                                      >  2:816028/1‑101                                          ACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCC                                                    >  1:12365/1‑101                                            CGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTA                                                  >  1:1069537/1‑101                                             GCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAA                                                 >  1:826244/1‑101                                              CTGGGATCTTCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                >  1:997508/1‑101                                                ggtATCTGCCATTGTCAGACATGTATCCCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                              <  2:947893/98‑1                                                GGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                              <  1:1049853/101‑1                                                  GATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGT                                            >  1:1340133/1‑101                                                      TGCCATTGTCAGACATGTATCCCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAA                                        <  2:301632/101‑1                                                       GCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG                                       <  2:652375/101‑1                                                            TGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAA                                  <  2:1626948/101‑1                                                              TCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAT                                <  2:145076/101‑1                                                                 GACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACC                             >  2:709842/1‑101                                                                 GACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACC                             <  2:602997/101‑1                                                                 GACATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACC                             <  2:597081/101‑1                                                                   CATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCT                           <  1:775180/101‑1                                                                    ATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTT                          >  2:473935/1‑101                                                                    ATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTT                          >  1:1523648/1‑101                                                                    ATGTATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTT                          >  1:1503886/1‑101                                                                       TATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTG                       >  1:444681/1‑101                                                                        ATACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGA                      <  1:964542/101‑1                                                                         TACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATTCCCTTTTGAA                     <  2:448405/101‑1                                                                         TACCCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA                     >  2:1560285/1‑101                                                                            CCCGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAG                  <  1:345278/101‑1                                                                              CGTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCG                >  1:1086143/1‑101                                                                               GTACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGC               >  2:1562891/1‑101                                                                                TACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCC              >  1:1700907/1‑101                                                                                TACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCC              >  1:713390/1‑101                                                                                 ACGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCT             <  2:1617497/101‑1                                                                                  CGTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTT            <  2:888230/101‑1                                                                                   GTCTTCCCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTG           >  2:1447219/1‑101                                                                                         CCGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCG     >  1:1008536/1‑101                                                                                          CGAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGT    >  1:223529/1‑101                                                                                           GAGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTG   >  2:478170/1‑101                                                                                            AGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGA  >  1:1285526/1‑101                                                                                            AGCGAAAATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGA  >  2:1153736/1‑101                                                                                                                                                                                                  GCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/336153‑336055 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGA  >  REL606/588495‑588587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.