Predicted mutation
evidence seq id position mutation freq annotation gene description
JC REL606 2,103,915 (CAGC)7→14 64.1% coding (182/216 nt) ECB_01992 → hypothetical protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2103888 =49 (0.520)78 (0.890) 38/74 NT 64.1% coding (155/216 nt) ECB_01992 hypothetical protein
?REL606 = 2103915 42 (0.480)coding (182/216 nt) ECB_01992 hypothetical protein

Only 100 of 109 total aligned reads displayed.

AGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2103940‑2103888
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCAC  <  REL606/2103915‑2103866
                                                                                                       
AGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                                                      >  1:5406651/1‑51
AGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                                                      >  1:987233/1‑51
AGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                                                      <  1:5405270/51‑1
AGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                                                      <  1:1739868/51‑1
  TACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                                    <  1:608502/51‑1
  TACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                                    <  1:2174107/51‑1
    CAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                                                  >  1:1202591/1‑51
     AAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                                                 >  1:4446360/1‑51
     AAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                                                 <  1:3096368/51‑1
     AAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                                                 <  1:2304464/51‑1
     AAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                                                 >  1:2609112/1‑51
       AATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                                                 >  1:6834078/1‑49
        ATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                                              <  1:10226633/51‑1
         TCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                                             <  1:8586524/51‑1
          CCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                                              >  1:1488531/1‑49
            ACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                                                     >  1:10377127/1‑40
             CATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                                         <  1:2020424/51‑1
              ATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                        >  1:930350/1‑51
              ATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                        <  1:8190704/51‑1
               TTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                                       <  1:7617206/51‑1
               TTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                                       <  1:1611772/51‑1
               TTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                                           >  1:6564873/1‑47
                TCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                                      >  1:6994286/1‑51
                 CACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                                     <  1:8746804/51‑1
                   CACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                                   <  1:320766/51‑1
                   CACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                                   <  1:9028671/51‑1
                     CCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                                 <  1:5545872/51‑1
                     CCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                                   <  1:5757717/49‑1
                      CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                <  1:8408637/51‑1
                      CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                <  1:1441319/51‑1
                      CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                <  1:7505419/51‑1
                      CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                <  1:4726136/51‑1
                      CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                <  1:10287104/51‑1
                      CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                <  1:4732352/51‑1
                       TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                               <  1:940143/51‑1
                       TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                               <  1:3123728/51‑1
                       TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                                >  1:8560273/1‑50
                        GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                              <  1:302847/51‑1
                        GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                              <  1:6277344/51‑1
                        GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                               >  1:5541211/1‑50
                        GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                                   >  1:5476020/1‑46
                        GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                                   >  1:9626191/1‑46
                         GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                             <  1:10033960/51‑1
                         GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                             <  1:9452067/51‑1
                         GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                             <  1:3104268/51‑1
                         GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                             <  1:10700683/51‑1
                          CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                            <  1:4312028/51‑1
                          CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                            <  1:2323898/51‑1
                           TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                           <  1:1724712/51‑1
                           TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                           <  1:8973719/51‑1
                            GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                           >  1:3707266/1‑50
                            GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                                      >  1:2362698/1‑39
                            GGCTGGCTGGCAGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                           >  1:9232791/1‑50
                             GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                         <  1:6120902/51‑1
                             GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCT                         <  1:9425833/51‑1
                              CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                        >  1:1455202/1‑51
                              CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG                        <  1:1210059/51‑1
                               TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGT                       <  1:4344694/51‑1
                               TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGT                       <  1:10779746/51‑1
                               TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                           >  1:8406991/1‑47
                                GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                           >  1:1591742/1‑46
                                 GCTGGCTGGCTGGCTGGCTGGCTGGCTTGCTGGCTGGCTGGCTGGCTGTGA                     <  1:10797193/51‑1
                                 GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTagct                     <  1:6011477/51‑5
                                 GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGA                     <  1:1571044/51‑1
                                 GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGA                     <  1:10779016/51‑1
                                  CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAA                    >  1:4408399/1‑51
                                  CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGC                              >  1:9376530/1‑41
                                  CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                               <  1:7246113/40‑1
                                    GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAAT                  <  1:6392701/51‑1
                                    GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                           >  1:7477059/1‑42
                                       TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAA                   >  1:2619638/1‑47
                                       TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAA                   >  1:2350634/1‑47
                                       TGGCTGGCTGACTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTA               >  1:10519616/1‑51
                                        GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAA              <  1:8938792/51‑1
                                         GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAAT             <  1:676454/51‑1
                                         GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAA              <  1:6427373/50‑1
                                          CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAAT             <  1:2330639/50‑1
                                           TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGC           <  1:2180324/51‑1
                                           TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGC           >  1:68426/1‑51
                                           TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGC           >  1:8438738/1‑51
                                           TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGG                           >  1:8856420/1‑35
                                            GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCA          <  1:5408943/51‑1
                                            GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCA          <  1:10128621/51‑1
                                            GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCT                >  1:6082303/1‑45
                                             GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAA         <  1:9217223/51‑1
                                             GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAA         <  1:6653903/51‑1
                                              CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAA        >  1:2037729/1‑51
                                               TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAAT       >  1:1536182/1‑51
                                                GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATT      >  1:8017115/1‑51
                                                GGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATT      >  1:9462326/1‑51
                                                  CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTC    <  1:10863530/51‑1
                                                  CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTC    >  1:7352820/1‑51
                                                  CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTC    >  1:10086401/1‑51
                                                  CTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTC    <  1:5077591/51‑1
                                                  CTGGCTGGCTGGCTGGCTGGCCGGCCGGCTGTGAAATCTAATGCAAATTTC    >  1:6131148/1‑51
                                                   TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCA   >  1:9293695/1‑51
                                                   TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCA   >  1:2881518/1‑51
                                                   TGGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCA   <  1:5391431/51‑1
                                                    GGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCAC  >  1:7019673/1‑51
                                                    GGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCAC  <  1:6670078/51‑1
                                                                                                       
AGTACAAAATCCACATTCACACCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2103940‑2103888
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgGCTGGCTGGCTGGCTGGCTGGCTGGCTGTGAAATCTAATGCAAATTTCAC  <  REL606/2103915‑2103866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.