BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC JC	REL606	490,484	IS150 (+) Δ1 bp :: +CATT	100%	intergenic (‑37/+24)	ybbN ← / ← ybbO	predicted thioredoxin domain‑containing protein/short chain dehydrogenase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	490484	490484	1	34 [0]	[0] 35	ybbN/ybbO	predicted thioredoxin domain‑containing protein/short chain dehydrogenase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 490483	0 (0.000)	21 (0.790)	17/278	NT	100%	intergenic (‑36/+25)	ybbN/ybbO	predicted thioredoxin domain‑containing protein/short chain dehydrogenase
*	?	REL606	= 2775877	NA (NA)	21 (0.790)	17/278	NT	100%	noncoding (1/1443 nt)	IS150	repeat region
*	?	REL606	490485 =	0 (0.000)	33 (1.260) +AAT	22/272	NT	100%	intergenic (‑38/+23)	ybbN/ybbO	predicted thioredoxin domain‑containing protein/short chain dehydrogenase
*	?	REL606	= 590472	NA (NA)	33 (1.260) +AAT	22/272	NT	100%	intergenic (+27/‑71)	insK‑1/hokE	IS150 putative transposase; b3558_1/toxic polypeptide, small

AGTGCTGGCTACGTTCAGACCAAAAATAGAACAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGTCACCAGCGTTACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCA  >  REL606/490336‑490625

aactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattg                                                                                                                                              >  1:587375‑M1/150‑150 (MQ=255)

   tgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattgggg                                                                                                                                           >  2:456529‑M1/147‑150 (MQ=255)

           gctgttacggaagatattgaataataaaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgcg                                                                                                                                   >  1:547954‑M1/139‑150 (MQ=255)

              gttacggaatatattgaatactacaacagcagaagaattagcctgcaattaaaaggtctgacttcaattgaatatcggaatcagacctatatgcctcgtgtttaaccgtccaactttttgggccgatgacacattGGGGCTTAAgcgcgc                                                                                                                                <  1:142379‑M1/15‑1 (MQ=255)

              gttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcctgtttaactgtccaacttcttggggtcagtacacattGGGGCTTAAgcgcgc                                                                                                                                <  1:433358‑M1/15‑1 (MQ=255)

                        atattgaatactacaacagcagaagaattagcctgcaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGGTGTTCAACTc                                                                                                                      <  1:591617‑M1/25‑1 (MQ=255)

                        atattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTc                                                                                                                      >  1:376067‑M1/126‑150 (MQ=255)

                        atattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTc                                                                                                                      <  2:599780‑M1/25‑1 (MQ=255)

                        atattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTc                                                                                                                      <  1:243502‑M1/25‑1 (MQ=255)

                        atattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTc                                                                                                                      <  1:119365‑M1/25‑1 (MQ=255)

                             gaatactacaacagcagaagaattagcctgaaattaataggtctgactccaattgattatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCAcccc                                                                                                                 >  1:84360‑M1/121‑150 (MQ=255)

                             gaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCAcccc                                                                                                                 <  1:62245‑M1/30‑1 (MQ=255)

                               atactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatccgccctatatgcctcgtgtttaactgtccaactttttggggtcagtacaaattTGAGCTTAAGTGCGCGCTTCGACTCACCCCTg                                                                                                               <  1:526723‑M1/32‑1 (MQ=255)

                                 actacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCa                                                                                                             <  1:60300‑M1/34‑1 (MQ=255)

                                                             attaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGgcag                                                                                 >  2:316133‑M1/89‑150 (MQ=255)

                                                                            tccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCAc                                                                  >  2:608836‑M1/74‑150 (MQ=255)

                                                                                  tgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTAc                                                            >  2:497263‑M1/68‑150 (MQ=255)

                                                                                  tgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTAc                                                            >  1:75194‑M1/68‑150 (MQ=255)

                                                                                  tgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTAc                                                            >  2:261318‑M1/68‑150 (MQ=255)

                                                                                       tataagagacagcctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGccc                                                       >  1:211081‑M1/63‑150 (MQ=255)

                                                                                       atcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGccc                                                       >  2:119715‑M1/63‑150 (MQ=255)

                                                                                           gaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGt                                                   <  1:648148‑M1/92‑1 (MQ=255)

                                                                                           gaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGt                                                   >  2:224451‑M1/59‑150 (MQ=255)

                                                                                            aatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGTc                                                  >  2:244191‑M1/58‑150 (MQ=255)

                                                                                               cagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGTCAcc                                               >  2:593357‑M1/55‑150 (MQ=255)

                                                                                                 gacctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCATCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGTCACCAg                                             <  2:415391‑M1/98‑1 (MQ=255)

                                                                                                 gacctatatgcctcgtgtttaactgtccaactttttgcggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGTCACCAg                                             <  2:79654‑M1/98‑1 (MQ=255)

                                                                                                   cctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAgcaccattactgcccctgtctcttata                                           <  2:211687‑M1/100‑13 (MQ=255)

                                                                                                   cctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGTCACCAGCg                                           <  2:223482‑M1/100‑1 (MQ=255)

                                                                                                          gcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGTCACCAGCGTTACCgg                                    <  2:552542‑M1/107‑1 (MQ=255)

                                                                                                               gtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGTCACCAGCGTTACCGGATAgc                               <  2:548628‑M1/112‑1 (MQ=255)

                                                                                                               gtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGTCACCAGCGTTACCGGATAgc                               <  2:547116‑M1/112‑1 (MQ=255)

                                                                                                                      actgtcgaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAAATCACCCCAGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGTCACCAGCGTTACCGGATAGCGCATCtt                        >  1:36752‑M1/32‑150 (MQ=255)

                                                                                                                                            gtacacgttGGGGCTTAAGCTCGCGCTTAAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCCCCCAAGCCCCATTACCGCCCAGGCCACCAGCGTTACCGCATAGCGCATCTTCGGCTGCTCGCTAATAAAAGCa  <  1:247567‑M1/141‑1 (MQ=255)

                                                                                                                                            gtacacattGGGGCTTAAGCGCGCGCTTCAACTCAGCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGTCACCAGCGTTACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCa  <  2:399654‑M1/141‑1 (MQ=255)

AGTGCTGGCTACGTTCAGACCAAAAATAGAACAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACGCGCCCCGGCAGCAGGCGCTTAAGCACCATTACTGCCCAGGTCACCAGCGTTACCGGATAGCGCATCTTCGGCTTCTCGCTAATAAAAGCA  >  REL606/490336‑490625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`