Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC JC REL606 490,484 IS150 (+) Δ1 bp :: +CATT 100% intergenic (‑37/+24) ybbN ← / ← ybbO predicted thioredoxin domain‑containing protein/short chain dehydrogenase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 490484 490484 1 34 [0] [0] 35 ybbN/ybbO predicted thioredoxin domain‑containing protein/short chain dehydrogenase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 4904830 (0.000)21 (0.790) 17/278 NT 100% intergenic (‑36/+25) ybbN/ybbO predicted thioredoxin domain‑containing protein/short chain dehydrogenase
?REL606 = 2775877 NA (NA)noncoding (1/1443 nt) IS150 repeat region
* ? REL606 490485 =0 (0.000)33 (1.260)
+AAT		 22/272 NT 100% intergenic (‑38/+23) ybbN/ybbO predicted thioredoxin domain‑containing protein/short chain dehydrogenase
?REL606 = 590472 NA (NA)intergenic (+27/‑71) insK‑1/hokE IS150 putative transposase; b3558_1/toxic polypeptide, small

TGCTTTTATTAGCGAGAAGCCGAAGATGCGCTATCCGGTAACGCTGGTGACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/490625‑490485
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCG  <  REL606/590472‑590325
                                                                                                                                             |||                                                                                                                                                    
TGCTTTTATTAGCGAGCAGCCGAAGATGCGCTATGCGGTAACGCTGGTGGCCTGGGCGGTAATGGGGCTTGGGGGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTTAAGCGCGAGCTTAAGCCCCAACGTGTAC                                                                                                                                                <  1:247567/150‑1
TGCTTTTATTAGCGAGAAGCCGAAGATGCGCTATCCGGTAACGCTGGTGACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGCTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTAC                                                                                                                                                <  2:399654/150‑1
                      AAGATGCGCTATCCGGTAACGCTGGTGACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCTGGGGTGATTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTCGACAGT                                                                                                                          >  1:36752/1‑150
                             GCTATCCGGTAACGCTGGTGACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACAC                                                                                                                   <  2:547116/150‑1
                             GCTATCCGGTAACGCTGGTGACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACAC                                                                                                                   <  2:548628/150‑1
                                  CCGGTAACGCTGGTGACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGC                                                                                                              <  2:552542/150‑1
                                         tataagagacagGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGG                                                                                                       <  2:211687/138‑1
                                         CGCTGGTGACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGG                                                                                                       <  2:223482/150‑1
                                           CTGGTGACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGATGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                                                                                                     <  2:415391/150‑1
                                           CTGGTGACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCGCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                                                                                                     <  2:79654/150‑1
                                             GGTGACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTG                                                                                                   >  2:593357/1‑150
                                                GACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATT                                                                                                >  2:244191/1‑150
                                                 ACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTC                                                                                               >  2:224451/1‑150
                                                 ACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTC                                                                                               <  1:648148/150‑1
                                                     GGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGctgtctcttata                                                                                           >  1:211081/1‑138
                                                     GGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGAT                                                                                           >  2:119715/1‑150
                                                          GTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCA                                                                                      >  2:261318/1‑150
                                                          GTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCA                                                                                      >  1:75194/1‑150
                                                          GTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCA                                                                                      >  2:497263/1‑150
                                                                GTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGA                                                                                >  2:608836/1‑150
                                                                               CTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAAT                                                                 >  2:316133/1‑150
                                                                                                           TGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGT                                     <  1:60300/150‑1
                                                                                                             CAGGGGTGAGTCGAAGCGCGCACTTAAGCTCAAATTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGGCGGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTAT                                   <  1:526723/150‑1
                                                                                                               GGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTC                                 <  1:62245/150‑1
                                                                                                               GGGGTGAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATAATCAATTGGAGTCAGACCTATTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTC                                 >  1:84360/1‑150
                                                                                                                    GAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATAT                            <  1:119365/150‑1
                                                                                                                    GAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATAT                            >  1:376067/1‑150
                                                                                                                    GAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATAT                            <  2:599780/150‑1
                                                                                                                    GAGTTGAAGCGCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATAT                            <  1:243502/150‑1
                                                                                                                    GAGTTGAACACCGCGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTGCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATAT                            <  1:591617/150‑1
                                                                                                                              GCGCGCTTAAGCCCCAATGTGTCATCGGCCCAAAAAGTTGGACGGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGAAGTCAGACCTTTTAATTGCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAAC                  <  1:142379/150‑1
                                                                                                                              GCGCGCTTAAGCCCCAATGTGTACTGACCCCAAGAAGTTGGACAGTTAAACAGGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAAC                  <  1:433358/150‑1
                                                                                                                                 CGCTTAAGCCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTTTATTATTCAATATCTTCCGTAACAGC               >  1:547954/1‑150
                                                                                                                                         CCCCAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCA       >  2:456529/1‑150
                                                                                                                                            CAATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTT    >  1:587375/1‑150
                                                                                                                                             AATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTC   >  2:247174/1‑150
                                                                                                                                             AATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTC   >  1:640186/1‑150
                                                                                                                                              ATGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCATTTCG  >  1:551136/1‑150
                                                                                                                                             |||                                                                                                                                                    
TGCTTTTATTAGCGAGAAGCCGAAGATGCGCTATCCGGTAACGCTGGTGACCTGGGCAGTAATGGTGCTTAAGCGCCTGCTGCCGGGGCGCGTGATGGACAAAATATTGCAGGGGTGAGTTGAAGCGCGCGCTTAAGCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/490625‑490485
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTCTTCTGCTGTTGTAGTATTCAATATATTCCGTAACAGCATCCTTCAGTTCG  <  REL606/590472‑590325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.