Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC REL606 3,766,595 IS150 (+) +3 bp 100% coding (1111‑1113/1206 nt) gltS ← glutamate transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 3766595 =0 (0.000)32 (1.210) 20/276 NT 100% coding (1113/1206 nt) gltS glutamate transporter
?REL606 = 3894996 NA (NA)noncoding (1443/1443 nt) IS150 repeat region
* ? REL606 = 37665970 (0.000)22 (0.820) 15/278 NT 100% coding (1111/1206 nt) gltS glutamate transporter
?REL606 3893554 = NA (NA)noncoding (1/1443 nt) IS150 repeat region

GAACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGACGCCTCC  >  REL606/3894851‑3895005
                                                                                                                                                 |         
gAACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAggct       >  2:324265/1‑147 (MQ=11)
     gAAGGATGCTGTTACGGAATATAGTGAATACTACAACATCAGAAGAATAAGCCTGATAGTAAAAGGTCTGACTCCGATTGAATATCGGAATAAGACCTATATGCCTCGTGTTTAACTGACCAACTTTTTGGGGTCAGTACAgtctccgaa  <  1:323630/150‑9 (MQ=11)
                                                                                                                                                 |         
GAACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGACGCCTCC  >  REL606/3894851‑3895005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.