| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| JC JC | REL606 | 1,354,425 | IS150 (–) +3 bp | 100% | coding (1053‑1055/1644 nt) | sapA ← | predicted antimicrobial peptide transporter subunit |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | 664688 = | NA (NA) | 21 (0.790) | 16/278 | NT | 100% | noncoding (1/1443 nt) | IS150 | repeat region |
| ? | REL606 | 1354425 = | 0 (0.000) | coding (1055/1644 nt) | sapA | predicted antimicrobial peptide transporter subunit | |||||
| * | ? | REL606 | = 666130 | NA (NA) | 20 (0.750) | 15/276 | NT | 100% | noncoding (1443/1443 nt) | IS150 | repeat region |
| ? | REL606 | = 1354427 | 0 (0.000) | coding (1053/1644 nt) | sapA | predicted antimicrobial peptide transporter subunit | |||||
AATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGT > REL606/1354280‑1354572 | aaTCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTGCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtg < 1:2690‑M2/150‑3 (MQ=255) cAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtac > 2:128176‑M2/1‑145 (MQ=255) cAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtac > 2:666362‑M2/1‑145 (MQ=255) cAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtac > 2:609190‑M2/1‑145 (MQ=255) cAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCACCCCCCACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtac > 1:388884‑M2/1‑145 (MQ=255) ttCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGTCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtactga > 1:87564‑M2/1‑142 (MQ=255) cttgccgagatgatcctgtatccatcatcagttgtgaagtagtgattcacgacttcaaagcgctttttaaaagggtccttttgctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagcacaTTGTCATAGGc < 1:124126‑M2/11‑1 (MQ=255) cgtgccgagatgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGc < 1:610369‑M2/11‑1 (MQ=255) tgtataagagacagctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGcc < 1:406773‑M2/12‑1 (MQ=255) gagatgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGa < 2:387549‑M2/17‑1 (MQ=255) tgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGAGgcg < 2:184892‑M2/21‑1 (MQ=255) tCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTGGCCTCGTTGtgtactgaccccaaaaagt > 2:196875‑M2/1‑131 (MQ=255) tcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCAcgaggctggc > 1:347778‑M2/127‑146 (MQ=255) tcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGAGgcgcgc > 2:361511‑M2/127‑150 (MQ=255) ctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacattgtcataggccctgtctcttataca > 2:407943‑M2/125‑137 (MQ=255) ggACTGGGTTTCCACGCCTGCGAACTTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAAATGTTCGTGAGATTTCGCCGTATTATATTCAGTAATTTTAGCCTCGTTGtctactgaccccataatgtttgac < 1:520279‑M2/150‑25 (MQ=255) ccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAAt < 2:29830‑M2/32‑1 (MQ=255) atcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAATag < 2:548058‑M2/34‑1 (MQ=255) gTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtactgaccccaaaaagttggacagttaaac > 2:92130‑M2/1‑118 (MQ=255) tCCACGCCTGCGAACGTGTGGGCACTCACAGTTTCATCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtactgaccccaaaaagttggacagttaaacac > 1:63351‑M2/1‑116 (MQ=255) tCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGATTTGAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTCGCCTCGTTGtgtactgaccccaaaaagttggacagttaaacac > 1:280182‑M2/1‑116 (MQ=255) cccaCGCCTGCGCACGTGTGGGCTACCACACTTTCAGCGTGAAATTTTCCCGCCCCATGGACATCAACTGTTCGCTCGATTTCGCTGGATTGTATTCAGTGATTTTAGCCTCGTTGtgtactgaccccaaaaagttggacagttaagcac > 1:29957‑M2/2‑116 (MQ=255) gttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGgccgc > 1:191892‑M2/110‑150 (MQ=255) gtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgtagtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGt > 1:38697‑M2/107‑150 (MQ=255) aaCGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTATATTCAGTAATTTTAGCCTCGTTGtgtactgaccccaaaaagttggacagttaaacacgaggcatatagg > 2:394932‑M2/1‑104 (MQ=255) attcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTAc > 2:624874‑M2/96‑150 (MQ=255) ggCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGATTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattc > 1:149442‑M2/1‑96 (MQ=255) ggCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattc > 2:463463‑M2/1‑96 (MQ=255) gacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCataat < 1:394531‑M2/61‑1 (MQ=255) gacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCataat < 2:627116‑M2/61‑1 (MQ=255) cccACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgat < 2:197709‑M2/150‑59 (MQ=255) acttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACcataatc > 2:395677‑M2/89‑149 (MQ=255) cttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAg < 1:623044‑M2/63‑1 (MQ=255) ccagtcgcttttcaaaagggtattgtggctttaacatatgaggtgctattccatttcatcggcaaacaagatggctgcagtacaTTGTCATAGGCCCACGAGGCGCGCGGGAAAATAGAGGCCGCCGTTTCAGCCGTACCCTAGTAGATg < 1:218223‑M2/66‑1 (MQ=255) gTTTCAGTGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGTGCGCTTTCGCCGGATTGTCTTCAGTAATTTTAGCCTCGTTGtgtactgactccaaaatgttggacagttaaacacgaggcatataggtcttattccgatattcaa < 1:264108‑M2/150‑65 (MQ=255) gTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaa < 2:117711‑M2/150‑65 (MQ=255) gCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggag < 2:235948‑M2/150‑71 (MQ=255) aGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaat > 1:117366‑M2/1‑66 (MQ=255) ttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGcc < 2:295101‑M2/96‑1 (MQ=255) ttacaccgtTCCGGCGATTTCGCCAGATTATATTCAGTAATTTTCGCCTCGTTGtgtactgaccccaaaaagttgtacagttaaacacgaggcatataggtctgattccgatattcaattggagtccgaccttttaatttcaggctaatt > 1:543328‑M2/5‑54 (MQ=255) attaggggctattccatttcatcgtccaacaaaatgggtgcagtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCa > 2:634058‑M2/48‑150 (MQ=255) gATTGTATTCAGTAATTTTAGCCTCGTTGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatat > 1:235280‑M2/1‑29 (MQ=255) gATTGTATTCAGTAATTTTAGCCTCGTTGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatat > 2:2703‑M2/1‑29 (MQ=255) aaatgggtgtagtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAgcgg < 2:38812‑M2/134‑1 (MQ=255) gCCTCGTTGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttc < 1:130019‑M2/150‑142 (MQ=255) gtacaTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCgg < 2:213929‑M2/145‑1 (MQ=255) cTCGTTGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcag > 2:130395‑M2/1‑7 (MQ=255) cGTTGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaatttgagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagtt > 1:25213‑M2/1‑5 (MQ=255) | AATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGT > REL606/1354280‑1354572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |