Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC REL606 1,354,425 IS150 (–) +3 bp 100% coding (1053‑1055/1644 nt) sapA ← predicted antimicrobial peptide transporter subunit

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 664688 =NA (NA)21 (0.790) 16/278 NT 100% noncoding (1/1443 nt) IS150 repeat region
?REL606 1354425 = 0 (0.000)coding (1055/1644 nt) sapA predicted antimicrobial peptide transporter subunit
* ? REL606 = 666130NA (NA)20 (0.750) 15/276 NT 100% noncoding (1443/1443 nt) IS150 repeat region
?REL606 = 1354427 0 (0.000)coding (1053/1644 nt) sapA predicted antimicrobial peptide transporter subunit

AACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/665986‑666130
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aCAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCCCACACGTTCGCAGGCGTGGAACCCCAGTCCACTGAAAACTGCCGAACTGATT  <  REL606/1354427‑1354280
                                                                                                                                                                                                                                                                                                     
AACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCAAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACG                                                                                                                                                 >  1:25213/1‑150
  CTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAG                                                                                                                                               >  2:130395/1‑150
    GAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGC                                                                                                                                             <  1:130019/150‑1
                        ATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCTAAAATTACTGAATACAATC                                                                                                                         >  1:235280/1‑150
                        ATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCTAAAATTACTGAATACAATC                                                                                                                         >  2:2703/1‑150
                                                 AATTAGCCTGAAATTAAAAGGTCGGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTACAACTTTTTGGGGTCAGTACACAACGAGGCGAAAATTACTGAATATAATCTGGCGAAATCGCCGGAACGGTgtaa                                                                                                >  1:543328/1‑146
                                                             ATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCT                                                                                    >  1:117366/1‑150
                                                                           CTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGC                                                                      <  2:235948/150‑1
                                                                                 TTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAAC                                                                <  2:117711/150‑1
                                                                                 TTGAATATCGGAATAAGACCTATATGCCTCGTGTTTAACTGTCCAACATTTTGGAGTCAGTACACAACGAGGCTAAAATTACTGAAGACAATCCGGCGAAAGCGCACGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACACTGAAAC                                                                <  1:264108/150‑1
                                                                                       ATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGG                                                          <  2:197709/150‑1
                                                                                           GAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCC                                                      >  2:463463/1‑150
                                                                                           GAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAATCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCC                                                      >  1:149442/1‑150
                                                                                                   CCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCTAAAATTACTGAATATAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCCCACACGTT                                              >  2:394932/1‑150
                                                                                                               GTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGATGAAACTGTGAGTGCCCACACGTTCGCAGGCGTGGA                                  >  1:63351/1‑150
                                                                                                               GTGTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCGAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTCAAATCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCCCACACGTTCGCAGGCGTGGA                                  >  1:280182/1‑150
                                                                                                               GTGCTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCTAAAATCACTGAATACAATCCAGCGAAATCGAGCGAACAGTTGATGTCCATGGGGCGGGAAAATTTCACGCTGAAAGTGTGGTAGCCCACACGTGCGCAGGCGTGGg                                  >  1:29957/1‑149
                                                                                                                 GTTTAACTGTCCAACTTTTTGGGGTCAGTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCCCACACGTTCGCAGGCGTGGAAC                                >  2:92130/1‑150
                                                                                                                         GTCAAACATTATGGGGTCAGTAGACAACGAGGCTAAAATTACTGAATATAATACGGCGAAATCTCACGAACATTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCCCACAAGTTCGCAGGCGTGGAAACCCAGTCC                        <  1:520279/150‑1
                                                                                                                              ACTTTTTGGGGTCAGTACACAACGAGGCCAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCCCACACGTTCGCAGGCGTGGAACCCCAGTCCACTGA                   >  2:196875/1‑150
                                                                                                                                         TCAGTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGACCACACGTTCGCAGGCGTGGAACCCCAGTCCACTGAAAACTGCCGAA        >  1:87564/1‑150
                                                                                                                                            GTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCCCACACGTTCGCAGGCGTGGAACCCCAGTCCACTGAAAACTGCCGAACTG     >  2:128176/1‑150
                                                                                                                                            GTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCCCACACGTTCGCAGGCGTGGAACCCCAGTCCACTGAAAACTGCCGAACTG     >  2:609190/1‑150
                                                                                                                                            GTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCCCACACGTTCGCAGGCGTGGAACCCCAGTCCACTGAAAACTGCCGAACTG     >  2:666362/1‑150
                                                                                                                                            GTACACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTGGGGGGTGGAAAATTTAACGCTGAAACTGTGGGTGCCCACACGTTCGCAGGCGTGGAACCCCAGTCCACTGAAAACTGCCGAACTG     >  1:388884/1‑150
                                                                                                                                               CACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGCACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCCCACACGTTCGCAGGCGTGGAACCCCAGTCCACTGAAAACTGCCGAACTGATT  <  1:2690/150‑1
                                                                                                                                                                                                                                                                                                     
AACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/665986‑666130
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aCAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTCGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGGTGCCCACACGTTCGCAGGCGTGGAACCCCAGTCCACTGAAAACTGCCGAACTGATT  <  REL606/1354427‑1354280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.