Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC REL606 4,092,861 IS150 (+) +3 bp 94.4% coding (87‑89/429 nt) yiiT → stress‑induced protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 3893554 =NA (NA)31 (1.180) 20/274 NT 93.8% noncoding (1/1443 nt) IS150 repeat region
?REL606 = 4092863 2 (0.080)coding (89/429 nt) yiiT stress‑induced protein
* ? REL606 = 3894996NA (NA)36 (1.380) 25/272 NT 94.6% noncoding (1443/1443 nt) IS150 repeat region
?REL606 4092861 = 2 (0.080)coding (87/429 nt) yiiT stress‑induced protein

GAACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGACGCCTCC  >  REL606/3894851‑3895005
                                                                                                                                                 |         
gAACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAggct       >  2:324265/1‑147 (MQ=11)
     gAAGGATGCTGTTACGGAATATAGTGAATACTACAACATCAGAAGAATAAGCCTGATAGTAAAAGGTCTGACTCCGATTGAATATCGGAATAAGACCTATATGCCTCGTGTTTAACTGACCAACTTTTTGGGGTCAGTACAgtctccgaa  <  1:323630/150‑9 (MQ=11)
                                                                                                                                                 |         
GAACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGACGCCTCC  >  REL606/3894851‑3895005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.