New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2100285 =	30 (1.120)	11 (0.490)	8/232	NT	22.4%	intergenic (+149/+124)	yegQ/ogrK	predicted peptidase/phage DNA‑binding transcriptional regulator
	?	REL606	= 2122430	51 (2.290)					coding (196/216 nt)	ECB_02013	conserved hypothetical protein; putative exported protein

TTTCGATTATTTTTCCCGGATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTAATTACATCCACATAATTTGC  >  REL606/2100141‑2100427                                                                                                                                                 |                                                                                                                                               taatccgctaagaggtggtactgaggccgagagtgtccacacagtgtccacagtagagtaacgtttaagggctttcagtggtaatttatgccgctcaaacccgcattgtaccgttgaaagcccctactggtgacaccctaaataTCCCtt                                                                                                                                           >  2:244594‑M1/145‑150 (MQ=255)     gATTATTTTTCCCGGATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTacac                                                                                                                                       <  2:323365/150‑1 (MQ=255)     gATTATTTTTCCCGGATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTacac                                                                                                                                       <  2:412510/150‑1 (MQ=255)     gATTATTTTTCCCGGATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTacac                                                                                                                                       <  2:502035/150‑1 (MQ=255)      attattTTTCCCGGATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAATGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACg                                                                                                                                      <  1:295617/150‑1 (MQ=255)                ccGGATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTAtttt                                                                                                                            <  2:670420/150‑1 (MQ=255)                  gtactgaggccgagagtgtccacacagtgtccacagtagagtaacgtttaagggctttcagtggtaatttatgccgctcaaacccgcattgtaccgttgaaagcccctactggtgacaccctaaataTCCCTTACACGGGCTTAtttttt                                                                                                                          <  2:10089‑M1/23‑1 (MQ=255)                             tCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATAc                                                                                                               <  2:577542/150‑1 (MQ=255)                               gagtgtccacacagtgtccacagtagagtaacgtttaagggctttcagtggtaatttatgccgctcaaacccgcattgtaccgttgaaagcccctactggtgacaccctaaataTCCCTTACACGGGCTTATTTTTTACGCGTAATACaa                                                                                                             <  2:288534‑M1/36‑1 (MQ=255)                                   gtccacacagtgtccacagtagagtaacgtttaagtgctttccgtggtaatttatgccgctcaaacccgcattgtaccgttgaaagcccctactggtcacaccctaaataTCCCTTAAACTGGCTTATTTTATACGCGTAATACATTGaa                                                                                                         <  1:356979‑M1/40‑1 (MQ=255)                                         aTCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATaaaa                                                                                                   >  1:601796/1‑150 (MQ=255)                                             caTACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGat                                                                                               >  2:539556/1‑150 (MQ=255)                                              gtccacagtagagtaacgtttaagggctttcagtggtaatttatgccgctcaaacccgcattgtaccgttgaaagcccctactggtgacaccctaaataTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGatt                                                                                              <  2:102884‑M1/51‑1 (MQ=255)                                                          ttattaAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCa                                                                                  >  2:540784/1‑150 (MQ=255)                                                             acgtttaagggctttcagtggtaatttatgccgctcaaacccgcattgtaccgttgaaagcccctactggtgacaccctaaataTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGt                                                                               <  2:499756‑M1/66‑1 (MQ=255)                                                                   ttATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCcacac                                                                         <  2:373767/150‑1 (MQ=255)                                                                   ttATCATCCGTTTCGCTGAAAAACATAACCCAAAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCcacac                                                                         <  1:31265/150‑1 (MQ=255)                                                                   aatggctttcagtggtaatttatgccgctcaaacccgcattgtacccttgaaagcccctactggtgacaccctaaataTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCcacac                                                                         >  1:168528‑M1/79‑150 (MQ=255)                                                                   aagggctttcagtggtaatttatgccgctcaaacccgcattgtaccgttgaaagcccctactggtgacaccctaaataTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCcacac                                                                         >  1:10056‑M1/79‑150 (MQ=255)                                                                   aagggctttcagtggtaatttatgccgctcaaacccgcattgtaccgttgaaagcccctactggtgacaccctaaataTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCcacac                                                                         >  2:46986‑M1/79‑150 (MQ=255)                                                                   aagggctttcagtggtaatttatgccgctcaaacccgcattgtaccgttgaaagcccctactggtgacaccctaaataTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCcacac                                                                         >  2:358007‑M1/79‑150 (MQ=255)                                                                     atcatcCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGGTTTATTTCTGGTCACGTCCACACAt                                                                       <  2:566642/150‑1 (MQ=255)                                                                     atcatcCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACAt                                                                       <  1:457799/150‑1 (MQ=255)                                                                     atcatcCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACAt                                                                       <  2:603566/150‑1 (MQ=255)                                                                                                       gcattgtaccgttgaaagcccctactggtgacaccctaaataTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAgg                                     >  1:102583‑M1/43‑150 (MQ=255)                                                                                                       gacagaGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAgg                                     <  2:259753/145‑1 (MQ=255)                                                                                                       aTGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAgg                                     >  2:26405/1‑150 (MQ=255)                                                                                                       aTGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAgg                                     >  2:233273/1‑150 (MQ=255)                                                                                                            aGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAggctgtc                                >  1:259013/1‑145 (MQ=255)                                                                                                            aGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCtt                                >  2:494057/1‑150 (MQ=255)                                                                                                              cTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATCCAATGAAATAAAAGGATTTATTTCTGTTCACGTCCACACATTGACCACATCGACAAAAAAGCCACTCGACTGAGTGGCTTcc                              >  1:515196/1‑150 (MQ=255)                                                                                                              cTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTcc                              <  1:232612/150‑1 (MQ=255)                                                                                                                             cctcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTAATTACAt               >  1:607489/1‑150 (MQ=255)                                                                                                                              actggtgacaccctaaataTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTAATTACATc              <  2:146816‑M1/131‑1 (MQ=255)                                                                                                                               tcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAACAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTAATTATATca             >  1:601349/1‑149 (MQ=255)                                                                                                                               tcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTATTTACCTcc             >  1:196584/1‑150 (MQ=255)                                                                                                                               tcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTAATTACATcc             >  2:548509/1‑150 (MQ=255)                                                                                                                               tcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTAATTACATcc             >  2:672166/1‑150 (MQ=255)                                                                                                                               tcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTAATTACATcc             >  2:327883/1‑150 (MQ=255)                                                                                                                               tcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTAATTACATca             >  2:12687/1‑149 (MQ=255)                                                                                                                                        ccctaaataTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTAATTACATCCACATAAttt    <  2:139543‑M1/141‑1 (MQ=255)                                                                                                                                         gtgtAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTAATTAAATACACATAATTtg   >  1:284465/1‑150 (MQ=255)                                                                                                                                          tgtAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTAATTACATCCACATAATTtgc  <  2:562075/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                               TTTCGATTATTTTTCCCGGATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTGTAATTACATCCACATAATTTGC  >  REL606/2100141‑2100427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.