Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC REL606 1,619,834 IS150 (–) +3 bp 100% intergenic (‑71/+139) hokD ← / ← ECB_01533 small toxic polypeptide/conserved hypothetical protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 664688 =NA (NA)19 (0.720) 16/274 NT 100% noncoding (1/1443 nt) IS150 repeat region
?REL606 1619834 = 0 (0.000)intergenic (‑71/+141) hokD/ECB_01533 small toxic polypeptide/conserved hypothetical protein
* ? REL606 = 666130NA (NA)46 (1.740) 29/276 NT 100% noncoding (1443/1443 nt) IS150 repeat region
?REL606 = 1619836 0 (0.000)intergenic (‑73/+139) hokD/ECB_01533 small toxic polypeptide/conserved hypothetical protein

GCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGT  >  REL606/664543‑664833
                                                                                                                                                 |                                                                                                                                                 
gcgcCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTAc                                                                                                                                               <  2:96274/150‑1 (MQ=255)
gcgcCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTAc                                                                                                                                               <  2:242056/150‑1 (MQ=255)
     gATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCAc                                                                                                                                          <  2:558074/150‑1 (MQ=255)
            gTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCAtttt                                                                                                                                   >  1:189458/1‑150 (MQ=255)
              tCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGCTTGCCACACGCTGCTGCTGACCGCCGGAAAGCCGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCACGACACGCTCACGCAGCTTTGTACTGCAACCATTttgc                                                                                                                                 >  1:48118/1‑149 (MQ=255)
                   aaaCAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGAc                                                                                                                            <  2:190000/150‑1 (MQ=255)
                               gagacagtctaTACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAATCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGaa                                                                                                                <  1:591207/140‑1 (MQ=255)
                                    gATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGCAACTTATTGGCGCGGGCAGAAAGCCCGAACCGCTCCAGCCGTTATGTACTTCACACACTTTGTGGGACGATGAAAGTGAATAAcc                                                                                                           >  1:35458/1‑150 (MQ=255)
                                    gATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcc                                                                                                           >  2:250928/1‑150 (MQ=255)
                                     aTCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGccc                                                                                                          >  2:72290/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAATCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGATATGGaactgtctc                                                                                                         >  2:592946/1‑143 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCCGCAGTTTTGTAATGCACCCATTTTGTTGGACGATGAAGTGGAATAGcccc                                                                                                         >  1:630144/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcccc                                                                                                         >  2:355987/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcccc                                                                                                         >  2:652625/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcccc                                                                                                         >  1:221249/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcccc                                                                                                         >  2:92173/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcccc                                                                                                         >  2:679400/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcccc                                                                                                         >  2:215923/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcccc                                                                                                         >  1:570862/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcccc                                                                                                         >  2:571649/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcccc                                                                                                         >  1:349636/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcccc                                                                                                         >  2:192853/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCCCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcccc                                                                                                         >  1:503211/1‑150 (MQ=255)
                                      tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGACGGAAAGATGCGCCGGAAACCTATTGGCGTGAGCAGAAAACCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTTTTGGACGATCAAATGGAATAGcccc                                                                                                         >  1:116509/1‑150 (MQ=255)
                                       ccATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCt                                                                                                        >  2:432235/1‑150 (MQ=255)
                                          tACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAat                                                                                                     <  2:221878/150‑1 (MQ=255)
                                          tACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAat                                                                                                     <  2:572527/150‑1 (MQ=255)
                                           acacAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAata                                                                                                    <  1:569986/150‑1 (MQ=255)
                                                     cgagcgaTTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                                                                                          <  2:504646/150‑1 (MQ=255)
                                                               ccACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCt                                                                                >  2:177875/1‑150 (MQ=255)
                                                                      ctgctactgACCGCGGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaaa                                                                         <  1:431010/150‑1 (MQ=255)
                                                                        gctgctgACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAg                                                                       >  1:21486/1‑150 (MQ=255)
                                                                                  gccgGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAg                                                             >  2:89846/1‑150 (MQ=255)
                                                                                   ccgGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGt                                                            <  2:512385/150‑1 (MQ=255)
                                                                                          gCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAAt                                                     <  1:89583/150‑1 (MQ=255)
                                                                                          gCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAAt                                                     <  2:21555/150‑1 (MQ=255)
                                                                                                  ggAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACtt                                             >  2:382561/1‑150 (MQ=255)
                                                                                                        ttATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAAc                                       <  2:116853/150‑1 (MQ=255)
                                                                                                             ggCGTGAGCAGCAAGCCCGACACGCTCCAGCCGTTTTGTACTGCACCCATTTTGTTCGACGATGAAATGGAATAGCCCCAAATATGTCAAAGCAAAAATACCCTTTTGAAAAGCGCCTTGAAGTCATGAATCACTACTTCACAACtgatg                                  >  1:169949/1‑150 (MQ=17)
                                                                                                                        ataGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTCTTGGACGACGAAATGGCATAGCCCCTAATATGTCAAAGCCAAAATACTCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACGACTTCACAACTGATGATGGTTACAgg                       <  1:662859/148‑1 (MQ=14)
                                                                                                                         atgCCCGACACGCTCCCGCAGTTTTGTACTGCAACCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGa                      <  1:501604/148‑1 (MQ=255)
                                                                                                                              cGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGatcatc                 <  1:177370/150‑1 (MQ=255)
                                                                                                                               gACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCAtct                <  1:406028/150‑1 (MQ=255)
                                                                                                                                     cTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCAc          >  1:407396/1‑150 (MQ=38)
                                                                                                                                     cTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCAc          >  1:428179/1‑150 (MQ=38)
                                                                                                                                             gTTTTGTACTGCACCCATTTTGTTGGACTTTTAACAAGAATATCCCCTAACATGTCAAAGCCAAAATACCGTTTTGAAAAGCGCCTTGAAGTCGTTAATCAATACTTCAGAGCTGATGACAGTGACAGGACCAATTCTGCACGGTTTgat  >  1:502312/1‑148 (MQ=2)
                                                                                                                                             gTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGgt  >  2:621165/1‑150 (MQ=32)
                                                                                                                                             gTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGgt  >  1:658434/1‑150 (MQ=32)
                                                                                                                                             gTTTTGTACTGCACCCATTTTGCTGGACGATGAAATGGAATAACGCCTAATATGTCAAAGCCAAAATACCCTTTTCAAAAGCGCCTTGAAGTAGTGAATCACTACTTCACAACGGGTTACGGTCACAGGACCATCTCGGCACGttatagt  >  1:178043/1‑145 (MQ=11)
                                                                                                                                             gTTTTGTACTGCACCAATTTTGTTGGACGATGAAATGGAATAGCACCTAATATGTCAAAGACAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATTATCACGGCACGTTTTTgt  >  1:643044/1‑150 (MQ=11)
                                                                                                                                                 |                                                                                                                                                 
GCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGT  >  REL606/664543‑664833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.