Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	REL606	3,841,192	IS150 (+) +3 bp	100%	coding (389‑391/1074 nt)	recF ←	recombination protein F

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 590471	NA (NA)	41 (1.540)	29/278	NT	100%	noncoding (1977/1977 nt)	IS150	repeat region
	?	REL606	3841192 =	0 (0.000)					coding (391/1074 nt)	recF	recombination protein F
*	?	REL606	= 3841194	0 (0.000)	31 (1.180)	25/274	NT	100%	coding (389/1074 nt)	recF	recombination protein F
	?	REL606	3893554 =	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

GAACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/590326‑590471 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGT  >  REL606/3841192‑3841327                                                                                                                                                                                                                                                                                            GAACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTT                                                                                                                                      <  2:619369/150‑1 GAACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTT                                                                                                                                      <  2:518204/150‑1      GAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGCATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGC                                                                                                                                 <  2:428636/150‑1      GAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGC                                                                                                                                 >  2:413211/1‑150      GAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGC                                                                                                                                 >  2:678149/1‑150              CTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTG                                                                                                                         >  1:403458/1‑150                GTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAG                                                                                                                       >  2:61506/1‑150                GTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAG                                                                                                                       >  1:576839/1‑150                GTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAG                                                                                                                       >  2:426643/1‑150                      GAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGT                                                                                                                 <  2:578528/150‑1                      GAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAAATGATTATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCTTTGAGTAAAGT                                                                                                                 <  1:455847/150‑1                          ATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAAC                                                                                                             >  1:329458/1‑150                          ATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAAC                                                                                                             >  2:189871/1‑150                            ATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCC                                                                                                           <  2:443041/150‑1                                  TACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCTACTTTTTGGGGTCAGTACAATTTTGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGG                                                                                                     >  1:445593/1‑150                                  TACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGATTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGG                                                                                                     >  1:423256/1‑150                                    CTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCG                                                                                                   >  1:211556/1‑150                                    CTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCG                                                                                                   <  2:37143/150‑1                                         ACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATC                                                                                              <  1:189330/150‑1                                          CAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCA                                                                                             >  2:97581/1‑150                                            GCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAAC                                                                                           <  2:264877/150‑1                                                      TAGCCTGAAATTAAAAGGTCTGACTACAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCA                                                                                 <  2:240114/150‑1                                                       AGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCAT                                                                                <  1:627201/150‑1                                                           TGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGG                                                                            <  2:424462/150‑1                                                           TGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGG                                                                            <  2:459894/150‑1                                                                      GGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTC                                                                 <  1:61320/150‑1                                                                      GGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTC                                                                 >  2:376026/1‑150                                                                                 AATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTAT                                                      >  1:84988/1‑150                                                                                    TGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCC                                                   <  2:365440/150‑1                                                                                    TGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCC                                                   <  2:212165/150‑1                                                                                           CGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGT                                            <  2:330393/150‑1                                                                                             tAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTAC                                          <  1:425435/149‑1                                                                                             GAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGCACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTAC                                          <  2:291400/150‑1                                                                                             GAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTCTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCCACTGCATTGGCATCACGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTAC                                          >  1:553858/1‑150                                                                                             GAAGCAGACCTATATGCAGCGTGTTTAACTGTCAAACTTTTTGGGGTCAGTACAATTTGGGGTCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCTCGACCTTATGCCCGTCTGTAC                                          <  1:233174/150‑1                                                                                                cagGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGT                                       <  2:452925/147‑1                                                                                                   GACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCtg                                    >  1:451643/1‑148                                                                                                   GACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGA                                    >  2:670338/1‑150                                                                                                     CCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATG                                  >  1:18145/1‑150                                                                                                     CCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATG                                  >  2:280518/1‑150                                                                                                                 GTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTG                      >  2:382898/1‑150                                                                                                                 GTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTG                      <  1:279719/150‑1                                                                                                                                     TTGGGGTCAGTACAATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGT  <  2:85234/150‑1                                                                                                                                                                                                                                                                                            GAACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/590326‑590471 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGT  >  REL606/3841192‑3841327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.