New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? GFP_Plasmid_Sequ 3001 =33048 (0.790)11 (0.000) 6/194 108.1 0.035% intergenic (–/–) –/– –/–
?GFP_Plasmid_Sequ 3021 = 31037 (0.760)intergenic (–/–) –/– –/–
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 32715 total aligned reads displayed.

CCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGG‑‑‑  >  GFP_Plasmid_Sequ/2921‑3115
                                                                                                    |                                                                                                 
 cgcAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAg                                                                                                  >  3:759811/1‑101 (MQ=255)
 cgcAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAg                                                                                                  >  3:1269890/1‑101 (MQ=255)
 cgcAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAg                                                                                                  >  3:1586693/1‑101 (MQ=255)
  gcAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAgt                                                                                                 <  3:871347/101‑1 (MQ=255)
        gAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGAct                                                                                           >  3:704791/1‑101 (MQ=255)
        gAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGAct                                                                                           <  1:1404532/101‑1 (MQ=255)
          aCGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGActct                                                                                         <  1:69583/101‑1 (MQ=255)
           cGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCta                                                                                        <  3:45060/101‑1 (MQ=255)
            gACCGCGCGCCGCGCGTCAGTGCGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCtag                                                                                       >  4:130975/1‑101 (MQ=255)
            gACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCtag                                                                                       >  1:1130153/1‑101 (MQ=255)
            gACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCtag                                                                                       <  3:1153459/101‑1 (MQ=255)
            gACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCtag                                                                                       <  1:203281/101‑1 (MQ=255)
            gACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCtag                                                                                       >  3:1095605/1‑101 (MQ=255)
               cGAGCGCAGCGCGTCCGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCtagtag                                                                                    >  4:40382/1‑101 (MQ=255)
               cGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCtagtag                                                                                    <  3:1327112/101‑1 (MQ=255)
                gAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTaga                                                                                   >  2:29796/1‑101 (MQ=255)
                gAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTaga                                                                                   <  3:92935/101‑1 (MQ=255)
                 aGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTagag                                                                                  >  1:236773/1‑101 (MQ=255)
                 aGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTagag                                                                                  >  3:731438/1‑101 (MQ=255)
                  gcgcAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTagaga                                                                                 <  3:336334/101‑1 (MQ=255)
                     cAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCg                                                                              >  3:1041692/1‑101 (MQ=255)
                         gAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCa                                                                          >  2:76033/1‑101 (MQ=255)
                         gAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCa                                                                          >  3:564698/1‑101 (MQ=255)
                         gAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCa                                                                          <  1:604493/101‑1 (MQ=255)
                           gTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCAcc                                                                        <  1:471874/101‑1 (MQ=255)
                            tCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCg                                                                       <  3:507964/101‑1 (MQ=255)
                            tCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCg                                                                       <  1:666859/101‑1 (MQ=255)
                                tGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGacaa                                                                   >  3:899665/1‑101 (MQ=255)
                                 gAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGacaaa                                                                  <  3:650292/101‑1 (MQ=255)
                                  aGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGacaaac                                                                 <  1:1370193/101‑1 (MQ=255)
                                   gCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGacaaaca                                                                <  1:266391/101‑1 (MQ=255)
                                     gAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAaacaac                                                              <  1:300990/101‑1 (MQ=255)
                                      aGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAacaaca                                                             <  1:1504774/101‑1 (MQ=255)
                                      aGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAacaaca                                                             <  3:814043/101‑1 (MQ=255)
                                       ggAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGCGACTCTAGCAGAGAGCGTTCACCGACAAACAACAg                                                            >  1:1031662/1‑101 (MQ=255)
                                         aaGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGAt                                                          <  3:1502449/101‑1 (MQ=255)
                                         aaGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGAt                                                          >  3:1067214/1‑101 (MQ=255)
                                           gCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATaa                                                        <  3:59263/101‑1 (MQ=255)
                                             cTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATaaaa                                                      <  1:825049/101‑1 (MQ=255)
                                               gCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACg                                                    <  3:622242/101‑1 (MQ=255)
                                               gCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACg                                                    <  1:1471072/101‑1 (MQ=255)
                                                 aGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGaa                                                  >  1:962790/1‑101 (MQ=255)
                                                  gCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGaaa                                                 <  3:1530817/101‑1 (MQ=255)
                                                  gCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGaaa                                                 <  3:278333/101‑1 (MQ=255)
                                                  gCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGaaa                                                 <  1:1538371/101‑1 (MQ=255)
                                                   cGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAg                                                <  3:1385391/101‑1 (MQ=255)
                                                    ggCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCTGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAgg                                               <  3:1560838/101‑1 (MQ=255)
                                                    ggCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAgg                                               <  3:986372/101‑1 (MQ=255)
                                                     gCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGc                                              <  3:957902/101‑1 (MQ=255)
                                                      ccGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGcc                                             <  3:676101/101‑1 (MQ=255)
                                                      ccGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGcc                                             >  1:1064348/1‑101 (MQ=255)
                                                       cGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGccc                                            <  1:537858/101‑1 (MQ=255)
                                                        gCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCa                                           >  3:194490/1‑101 (MQ=255)
                                                        gCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCa                                           <  3:1181920/101‑1 (MQ=255)
                                                        gCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCa                                           >  2:133808/1‑101 (MQ=255)
                                                         ctactaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAg                                          <  3:1123874/101‑1 (MQ=255)
                                                         ctactaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAg                                          >  1:636506/1‑101 (MQ=255)
                                                         ctactaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAg                                          >  3:1357980/1‑101 (MQ=255)
                                                          tactaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGt                                         <  3:476700/101‑1 (MQ=255)
                                                           actaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTc                                        <  1:994628/101‑1 (MQ=255)
                                                           actaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTc                                        >  3:1415034/1‑101 (MQ=255)
                                                            ctaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCt                                       <  3:424031/101‑1 (MQ=255)
                                                            ctaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCt                                       <  1:1569630/101‑1 (MQ=255)
                                                             taGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCtt                                      >  3:393590/1‑101 (MQ=255)
                                                              aGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCttt                                     <  3:16698/101‑1 (MQ=255)
                                                               gTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTc                                    <  1:503842/101‑1 (MQ=255)
                                                                tatattAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCg                                   >  1:1268832/1‑101 (MQ=255)
                                                                tatataAACGCAGAACGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGGGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCg                                   <  3:220665/101‑1 (MQ=255)
                                                                tatataAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCg                                   <  1:1000057/101‑1 (MQ=255)
                                                                tatataAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCg                                   <  1:5716/101‑1 (MQ=255)
                                                                   ataAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACt                                >  3:534789/1‑101 (MQ=255)
                                                                   ataAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACt                                <  3:2489/101‑1 (MQ=255)
                                                                     aaaCGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGa                              <  1:337905/101‑1 (MQ=255)
                                                                      aaCGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAg                             <  1:1095771/101‑1 (MQ=255)
                                                                         gCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCt                          <  3:1013291/101‑1 (MQ=255)
                                                                          cAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCtt                         <  1:1197203/101‑1 (MQ=255)
                                                                           aGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCttt                        >  1:929185/1‑101 (MQ=255)
                                                                           aGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAAC‑ACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTc                       <  1:406973/101‑1 (MQ=255)
                                                                            gAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTc                       <  1:727775/101‑1 (MQ=255)
                                                                             aaaGGCCCACCCGAAGGTGAGCCAGCGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCg                      <  1:1234011/101‑1 (MQ=255)
                                                                              aaGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGt                     <  3:1443544/101‑1 (MQ=255)
                                                                               aGGCCCGCCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGtt                    <  1:130326/101‑1 (MQ=255)
                                                                               aGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGtt                    <  3:1474532/101‑1 (MQ=255)
                                                                               aGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGtt                    <  1:793186/101‑1 (MQ=255)
                                                                                 gCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGtttt                  <  3:1299356/101‑1 (MQ=255)
                                                                                  cccACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTa                 >  3:121187/1‑101 (MQ=255)
                                                                                  cccACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTa                 <  3:308286/101‑1 (MQ=255)
                                                                                   ccACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTAt                <  1:170273/101‑1 (MQ=255)
                                                                                   ccACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTAt                <  3:787888/101‑1 (MQ=255)
                                                                                     aCCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTAttt              <  1:37092/101‑1 (MQ=255)
                                                                                       ccGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGa            >  1:1436422/1‑101 (MQ=255)
                                                                                       ccGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGa            <  1:89861/101‑1 (MQ=255)
                                                                                        cGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGAt           <  1:1338398/101‑1 (MQ=255)
                                                                                         gAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATg          >  3:843217/1‑101 (MQ=255)
                                                                                         gAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATg          >  3:1242472/1‑101 (MQ=255)
                                                                                          aaGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGc         >  3:367066/1‑101 (MQ=255)
                                                                                          aaGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGc         >  1:864833/1‑101 (MQ=255)
                                                                                             gTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTg      <  1:1163970/101‑1 (MQ=255)
                                                                                             gTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTg      >  1:760732/1‑101 (MQ=255)
                                                                                                 gCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGctc  <  1:441486‑M2/101‑4 (MQ=255)
                                                                                                    |                                                                                                 
CCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGG‑‑‑  >  GFP_Plasmid_Sequ/2921‑3115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.