New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? GFP_Plasmid_Sequ 3001 =33048 (0.790)11 (0.000) 6/194 108.1 0.035% intergenic (–/–) –/– –/–
?GFP_Plasmid_Sequ 3021 = 31037 (0.760)intergenic (–/–) –/– –/–
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 33331 total aligned reads displayed.

GAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTT  >  GFP_Plasmid_Sequ/2901‑3101
                                                                                                    |                                                                                                    
gagtgagCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAg                                                                                                      <  3:1090904/101‑1 (MQ=255)
 agtgagCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAgg                                                                                                     <  3:126541/101‑1 (MQ=255)
 agtgagCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAgg                                                                                                     <  3:446951/101‑1 (MQ=255)
  gtgagCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGc                                                                                                    <  1:64177/101‑1 (MQ=255)
   taagCTAATCCCCCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGGGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGcc                                                                                                   <  1:1131202/99‑1 (MQ=255)
    gagCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGccc                                                                                                  >  1:1195812/1‑101 (MQ=255)
     agCTGATCCCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCa                                                                                                 <  1:1364800/101‑1 (MQ=255)
     agCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCa                                                                                                 <  1:672655/101‑1 (MQ=255)
      gCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCAc                                                                                                <  3:390894/101‑1 (MQ=255)
      gCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCAc                                                                                                <  1:224272/101‑1 (MQ=255)
      gCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCAc                                                                                                <  1:832205/101‑1 (MQ=255)
        tGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCAccc                                                                                              >  3:277493/1‑101 (MQ=255)
        tGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCAccc                                                                                              >  3:166301/1‑101 (MQ=255)
        tGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCAccc                                                                                              >  1:1559259/1‑101 (MQ=255)
         gATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCg                                                                                             <  3:615921/101‑1 (MQ=255)
          aTACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGa                                                                                            <  3:1381886/101‑1 (MQ=255)
           tACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGaa                                                                                           <  3:808261/101‑1 (MQ=255)
            aCCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAg                                                                                          <  3:644310/101‑1 (MQ=255)
             ccGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAgg                                                                                         >  3:120850/1‑101 (MQ=255)
                cTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGa                                                                                      <  3:1006663/101‑1 (MQ=255)
                cTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGa                                                                                      <  3:699334/101‑1 (MQ=255)
                 tCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAg                                                                                     >  3:1292087/1‑101 (MQ=255)
                  cgccgcAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGc                                                                                    >  1:481042/1‑101 (MQ=255)
                  cgccgcAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGc                                                                                    >  3:590245/1‑101 (MQ=255)
                  cgccgcAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGc                                                                                    >  3:1442244/1‑101 (MQ=255)
                    ccgcAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCa                                                                                  <  3:1236169/101‑1 (MQ=255)
                      gcggCCGAACGCCCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCGGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAgt                                                                                <  1:286047/98‑1 (MQ=255)
                      gcAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAgt                                                                                <  1:581218/101‑1 (MQ=255)
                      gcAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAgt                                                                                <  3:920424/101‑1 (MQ=255)
                       cAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAgtg                                                                               <  3:1322843/101‑1 (MQ=255)
                        ggCCGAACGCCCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGGAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAgtgt                                                                              <  1:1229014/100‑1 (MQ=255)
                        aGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAgtgt                                                                              <  1:1163482/101‑1 (MQ=255)
                        aGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAgtgt                                                                              <  3:1559552/101‑1 (MQ=255)
                           cGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGAc                                                                           <  1:1429283/101‑1 (MQ=255)
                                gACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCtag                                                                      >  3:949166/1‑101 (MQ=255)
                                 acggAGCGCGGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCtagt                                                                     <  3:1119064/98‑1 (MQ=255)
                                 aCCGAGCGCAGCGAGTCAGTGAGTGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCtagt                                                                     <  1:733488/101‑1 (MQ=255)
                                    gAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTaga                                                                  <  3:835247/101‑1 (MQ=255)
                                    gAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTaga                                                                  <  1:38650/101‑1 (MQ=255)
                                      gcgcAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTagaga                                                                <  1:1334489/101‑1 (MQ=255)
                                      gcgcAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTagaga                                                                <  3:1586567/101‑1 (MQ=255)
                                       cgcAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTagagag                                                               <  3:219190/101‑1 (MQ=255)
                                       cgcAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTagagag                                                               <  1:61195/101‑1 (MQ=255)
                                         cAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCg                                                             >  3:561084/1‑101 (MQ=255)
                                           gCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGtt                                                           >  3:472238/1‑101 (MQ=255)
                                            cGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTc                                                          >  3:671209/1‑101 (MQ=255)
                                            cGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTc                                                          <  1:15269/101‑1 (MQ=255)
                                             gAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCa                                                         >  3:725570/1‑101 (MQ=255)
                                             gAGTAAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCa                                                         <  1:936490/101‑1 (MQ=255)
                                              aGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCAc                                                        <  3:1178234/101‑1 (MQ=255)
                                              aGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCAc                                                        <  1:96756/101‑1 (MQ=255)
                                                tCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCg                                                      <  1:1463092/101‑1 (MQ=255)
                                                  aGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGac                                                    <  1:1399434/101‑1 (MQ=255)
                                                  aGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGac                                                    <  3:193252/101‑1 (MQ=255)
                                                   gTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGaca                                                   <  1:191525/101‑1 (MQ=255)
                                                    ggAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGacaa                                                  <  3:781581/100‑1 (MQ=255)
                                                       gCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGacaaaca                                               <  1:1263587/101‑1 (MQ=255)
                                                         gAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAaacaac                                             <  3:1473242/101‑1 (MQ=255)
                                                             aaGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGAt                                         >  1:510919/1‑101 (MQ=255)
                                                              aGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATa                                        <  3:333715/101‑1 (MQ=255)
                                                              aGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATa                                        <  1:799214/101‑1 (MQ=255)
                                                                ccTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATaaa                                      >  1:1096543/1‑101 (MQ=255)
                                                                  tggaGCGCCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAAc                                    <  3:1147009/98‑1 (MQ=255)
                                                                  tgaaGCTGCAGCTACTAGTATATAAACGCAGAAATGCCCATCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAAc                                    <  1:1590346/98‑1 (MQ=38)
                                                                  tGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAAc                                    <  1:31924/101‑1 (MQ=255)
                                                                  tGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAAc                                    <  3:531744/101‑1 (MQ=255)
                                                                      gCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGaaa                                <  3:305076/101‑1 (MQ=255)
                                                                       cGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAg                               <  3:247363/101‑1 (MQ=255)
                                                                         gCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGc                             >  2:57842/1‑101 (MQ=255)
                                                                         gCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGAGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGc                             >  3:503059/1‑101 (MQ=255)
                                                                          ccGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGcc                            <  3:1500424/101‑1 (MQ=255)
                                                                           cGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGccc                           >  1:998580/1‑101 (MQ=255)
                                                                            gCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCa                          >  1:768459/1‑101 (MQ=255)
                                                                             ctactaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAg                         <  4:31495/101‑1 (MQ=255)
                                                                             ctactaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAg                         >  1:421225/1‑101 (MQ=255)
                                                                             ctactaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAg                         >  3:364031/1‑101 (MQ=255)
                                                                              tactaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGt                        >  1:903386/1‑101 (MQ=255)
                                                                              tactaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCACAGt                        <  3:862875/101‑1 (MQ=255)
                                                                                ctaGTATCTACACGCCGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCt                      >  4:11112/1‑101 (MQ=255)
                                                                                ctaGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCt                      >  3:1035856/1‑101 (MQ=255)
                                                                                   gTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCTAGTGTGACTCTAGTAGAGAGCGTTCCCCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTc                   <  1:252269/101‑1 (MQ=255)
                                                                                   gTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTc                   <  3:75387/101‑1 (MQ=255)
                                                                                    tatataAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCg                  <  1:1000057/101‑1 (MQ=255)
                                                                                     atataAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGa                 >  1:1494206/1‑101 (MQ=255)
                                                                                      tataAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGAc                <  3:1411108/101‑1 (MQ=255)
                                                                                       ataAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACt               <  1:1030788/101‑1 (MQ=255)
                                                                                        taAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTg              >  1:452368/1‑101 (MQ=255)
                                                                                         aaaCGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGa             >  1:545847/1‑101 (MQ=255)
                                                                                         aaaCGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGa             <  3:1061210/101‑1 (MQ=255)
                                                                                          aaCGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAg            >  1:1300079/1‑101 (MQ=255)
                                                                                           aCGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGc           <  1:351625/101‑1 (MQ=255)
                                                                                            cGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGcc          >  2:149135/1‑101 (MQ=255)
                                                                                             gCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCt         <  1:1062378/101‑1 (MQ=255)
                                                                                               aGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCttt       <  3:1529083/101‑1 (MQ=255)
                                                                                                gAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTc      <  3:889703/101‑1 (MQ=255)
                                                                                                gAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTc      <  3:420184/101‑1 (MQ=255)
                                                                                                 aaaGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCg     <  1:702177/101‑1 (MQ=255)
                                                                                                  aaGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGt    >  3:1352766/1‑101 (MQ=255)
                                                                                                   aGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGtt   >  3:977025/1‑101 (MQ=255)
                                                                                                   aGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGtt   <  1:869936/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTT  >  GFP_Plasmid_Sequ/2901‑3101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.