New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ADP1‑WT 1722721 =0 (0.000)36 (2.070) 30/250 0.2 100% coding (1536/2397 nt) quiA quinate/shikimate dehydrogenase [pyrroloquinoline‑quinone] (NAD(P)‑independent quinate dehydrogenase)
?pYTK001_with_tdk_kan = 1688 0 (NA)intergenic (–/+123) –/CamR –/–

ATCAGACTCTTTAAGTGTCTGATTACCAATTTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ADP1‑WT/1722838‑1722721
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAGTGAAGGAGAAAA  <  pYTK001_with_tdk_kan/1688‑1573
                                                                                                                                                                                                                                          
ATCAGACTCTTTAAGTGTCTGATTACCAATTTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCC                                                                                                              >  1:48888/1‑126
 TCAGACTCTTTAAGTGTCTGATTACCAATTTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCG                                                                                                             >  2:47821/1‑126
    GACTCTTTAAGTGTCTGATTACCAATTTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCAT                                                                                                          <  1:18236/126‑1
        CTTTAAGTGTCTGATTACCAATTTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTT                                                                                                      <  2:111902/126‑1
         TTTAAGTGTCTGATTACCAATTTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTA                                                                                                     >  2:369863/1‑126
         TTTAAGTGTCTGATTACCAATTTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTA                                                                                                     >  2:168363/1‑126
          TTAAGTGTCTGATTACCAATTTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAG                                                                                                    <  2:48825/126‑1
                        ACCAATTTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAG                                                                                      >  1:413493/1‑126
                        ACCAATTTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAG                                                                                      >  2:8396/1‑126
                          CAATTTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCA                                                                                    >  1:469494/1‑126
                              TTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAA                                                                                <  1:143591/126‑1
                              TTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAA                                                                                >  1:281340/1‑126
                               TGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAA                                                                               <  2:129761/126‑1
                                GCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAAT                                                                              <  2:412895/126‑1
                                             TGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTT                                                                 >  2:177859/1‑126
                                                ACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATAACAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATT                                                              >  1:277407/1‑126
                                                       TGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCA                                                       >  2:201379/1‑126
                                                          GTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGA                                                    >  2:246665/1‑126
                                                          GTTTTACTGTATTGTTCGCCAGGAATAACAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGA                                                    <  2:276991/126‑1
                                                           TTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGAT                                                   <  1:47881/126‑1
                                                                  GTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAAT                                            <  2:280916/126‑1
                                                                    ATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATAC                                          <  1:247045/126‑1
                                                                     TTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACC                                         >  1:377554/1‑126
                                                                     TTGTTCGCCAGGAATATCAGCCACTTTAATTAGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACC                                         >  1:416661/1‑126
                                                                      TGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCA                                        >  2:306983/1‑126
                                                                      TGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCA                                        >  1:46482/1‑126
                                                                          CGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATT                                    <  2:207364/126‑1
                                                                          CGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATT                                    <  1:15285/126‑1
                                                                              AGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTG                                >  1:355707/1‑126
                                                                                  ATATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAA                            >  2:93799/1‑126
                                                                                   TATCAGCCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAA                           <  2:363910/126‑1
                                                                                         CCACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTT                     <  2:468816/126‑1
                                                                                          CACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTT                    <  1:370382/126‑1
                                                                                          CACTTTAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTT                    <  1:168612/126‑1
                                                                                               TAATTGGCTGCTCGATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTA               <  1:8407/126‑1
                                                                                                            GATCACTTTACCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAGTGAAGGAGAAAA  <  2:46426/126‑1
                                                                                                                                                                                                                                          
ATCAGACTCTTTAAGTGTCTGATTACCAATTTGCGGCATTTCAACTGAACGCGGCTGAGTTTTACTGTATTGTTCGCCAGGAATATCAGCCACTTTAATTGGCTGCTCGATCACTTTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ADP1‑WT/1722838‑1722721
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCAGCTCCGCATGCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAGTGAAGGAGAAAA  <  pYTK001_with_tdk_kan/1688‑1573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.