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---+ Retired Protocols

   * [[ProtocolsBTKDesignPart][Designing a new part]]<br> Designing a new part for use in the BTK
   * [[BroadHostRangeToolkit][Bee Microbiome Toolkit (BTK)]] <br />Golden Gate part kit for work in non-model bacterial species

---++ Retired on May 23, 2014

   * [[ProceduresCrushSoakPolyacrylamideGelElution][Crush-Soak Elution]]<br>Recover DNA or RNA samples from polyacrylamide gels.
   * [[ProtocolsEthanolPrecipitation][Ethanol Precipitation]]<br>Precipitate DNA or RNA samples to remove salts and concentrate. 
   * [[ProceduresPrimerExtension][Primer Extension]]
   * [[ProceduresTotalAlkalineDigestofEmbeddedRNAlinkages][Total Alkaline Digest of Embedded RNA linkages]]
   * [[ProtocolsSettingUpAutotools][Setting up compilation using Autotools]]
   * [[ProtocolsBreseqResults][Interpreting <i>breseq</i> output.]] <br>How to interpret, check, and manually correct <i>breseq</i> results.
   * [[ProceduresPulsedFieldGelElectrophoresisMapping][Pulsed-Field Gel Electrophoresis (PFGE) Mapping]]<br>To validate large-scale chromosomal rearrangements, deletions, and duplications.
   * [[ProtocolsUsingAPE][Using APE]]
   * [[AttritionQuantification][Measuring the Rate of Attrition in frozen or cooled samples]]
   * [[ProtocolsSoilGenomicDNAPrep][Extracting Clean Metagenomic DNA From Soil]]

---++ Previously Retired
   * [[ProceduresEColiMicroarray][E. coli Microarray]]
   * [[ProceduresVeracodeGoldenGateGenotyping][Veracode Golden Gate Genotyping]]
   * [[Trash.LabProceduresFluctuationTest][Phage T5 Fluctuation Test]]
   * [[ProceduresEvolvabilityCompetitions][96-Well Microplate Competitions]] 
      * [[ProcedureMixingandPlatingChecklist][Plating Checklist]]
   * [[ProceduresChangingEnvironmentLongTerm][Changing Environment Long Terms]]
   * [[ProceduresMarkerDivergenceLongTerm][Marker Divergence Long Terms]] 
      * [[ProcedurePinTool][Using the Deep 96-Well Pin Tool]]
   * [[ProceduresPopulationMetagenomicsLongTerm][Population Metagenomics Long Term]]
   * [[ProceduresGenomeMinimizationGrowthDeathAssays][Genome Minimization Growth/Death Assays]]
   * [[ProceduresASKAEvolutionExperiment][ASKA Strain Evolution Experiment]]
Topic revision: r10 - 2025-02-10 - JeffreyBarrick