Quantitative PCR (qPCR)

Quantitative PCR (qPCR) is a tool routinely used for the quantitation of target nucleic acid sequences. If it is your first time performing qPCR, we recommend reading this in-depth document before you begin. Protocols for qPCR vary substantially depending on the question being asked and the input nucleic acid used. This flowchart can help you decide which qPCR approach will work best for you.

Protocols for each main goal can be found at the links below:

• Comparative RT-qPCR
• Absolute qPCR for plasmid copy number