TWiki's Lab webThe Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise.https://barricklab.org/twiki/bin/view/LabCopyright 2026 by contributing authors2026-08-18T15:14:18ZWebStatisticshttps://barricklab.org/twiki/bin/view/Lab/WebStatistics2026-08-18T15:14:18ZStatistics for Lab Web Month: Topic views: Topic saves: File uploads: Most popular topic views: Top contributors for topic save and... (last changed by TWikiGuest)TWikiGuestPublicationListhttps://barricklab.org/twiki/bin/view/Lab/PublicationList2026-08-16T21:23:54ZBarrick Lab :: Publications Barrick Lab Publications on Google Scholar Legend: Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsConjugationhttps://barricklab.org/twiki/bin/view/Lab/ProtocolsConjugation2026-07-29T17:18:46ZGeneral Conjugation Protocol Return to BTK Page Conjugation is a reliable, robust method to transfer plasmids between bacteria. This is a general purpose protocol... (last changed by IraZibbu)IraZibbuProtocolListhttps://barricklab.org/twiki/bin/view/Lab/ProtocolList2026-05-27T21:56:24ZBarrick Lab :: Laboratory Protocols Updating Protocols How to Create a Protocol Best practices for designing a protocol and for putting it on the lab Wiki.... (last changed by JeffreyBarrick)JeffreyBarrickTestTopichttps://barricklab.org/twiki/bin/view/Lab/TestTopic2026-05-18T09:58:09Zasdasdf JeffreyBarrick 20 Jun 2009 (last changed by TestUser)TestUserWebHomehttps://barricklab.org/twiki/bin/view/Lab/WebHome2026-05-15T10:17:16Z.within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick)JeffreyBarrickReferencePlasmidshttps://barricklab.org/twiki/bin/view/Lab/ReferencePlasmids2026-05-13T15:26:23ZCommonly Used Plasmids plasmid markers origin host ref description pCA24N CamR Kitagawa2005 ASKA collection vector pKD... (last changed by IraZibbu)IraZibbuListofBioinformaticshttps://barricklab.org/twiki/bin/view/Lab/ListofBioinformatics2026-05-13T15:17:01ZBioinformatics Master List This page is a master list of bioinformatics software frequently used in the lab, and their purposes. It also includes additional notes... (last changed by IraZibbu)IraZibbuProtocolsBTKAssembleSingleTUPlasmidhttps://barricklab.org/twiki/bin/view/Lab/ProtocolsBTKAssembleSingleTUPlasmid2026-05-13T15:11:40ZBack to Golden Gate Protocols First Stage Plasmid (Transcriptional Unit) Assembly Once you have all your desired part plasmids built you can assemble them into Transcriptional... (last changed by AnthonyVandieren)AnthonyVandierenStrain_side_noteshttps://barricklab.org/twiki/bin/view/Lab/Strain_side_notes2026-05-13T15:01:32ZCommonly Used E. coli Strains OpenWetWare offers a comprehensive repository of the genotypes of the most commonly used E. coli strains. The following table points... (last changed by IraZibbu)IraZibbuLabPersonnelhttps://barricklab.org/twiki/bin/view/Lab/LabPersonnel2026-05-01T12:29:39ZBarrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick)JeffreyBarrickUTiGEMTeamhttps://barricklab.org/twiki/bin/view/Lab/UTiGEMTeam2026-04-30T10:09:33ZThe University of Texas at Austin :: iGEM Team The Barrick lab sponsored and mentored the UT Austin iGEM team from 2012 2025 when we were located at The University... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsElectrocompetentCellshttps://barricklab.org/twiki/bin/view/Lab/ProtocolsElectrocompetentCells2026-04-29T13:52:59ZElectrocompetent E. coli Making Electrocompetent E. coli Cells (small batch) This procedure makes enough electrocompetent cells for 2 3 transformations. 1 Grow... (last changed by IraZibbu)IraZibbuProtocols16SSequencinghttps://barricklab.org/twiki/bin/view/Lab/Protocols16SSequencing2026-04-28T19:00:14Z16S rRNA Sequencing to Identify Unknown Microbes Ever wonder what that contaminant in your culture is? Need to accurately identify an environmental isolate? Overview... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsRecipesABMShttps://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesABMS2026-04-28T00:45:40ZABMS: Acinetobacter Minimal Succinate Courtesy of the Averhoff lab, with modifications for available reagents. To make standard ABMS, combine the following pre sterilized... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsRecipesDavisMingiolihttps://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesDavisMingioli2026-04-22T18:39:45ZDM: Davis Mingioli Growth medium used by the long term E. coli evolution experiment. 0.5L 1L 4.5L 5L Component MW 2.67 g... (last changed by JeffreyBarrick)JeffreyBarrick
TWiki's Lab webThe Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise.https://barricklab.org/twiki/bin/view/LabCopyright 2026 by contributing authors2026-08-18T15:14:18ZWebStatisticshttps://barricklab.org/twiki/bin/view/Lab/WebStatistics2026-08-18T15:14:18ZStatistics for Lab Web Month: Topic views: Topic saves: File uploads: Most popular topic views: Top contributors for topic save and... (last changed by TWikiGuest)TWikiGuestPublicationListhttps://barricklab.org/twiki/bin/view/Lab/PublicationList2026-08-16T21:23:54ZBarrick Lab :: Publications Barrick Lab Publications on Google Scholar Legend: Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsConjugationhttps://barricklab.org/twiki/bin/view/Lab/ProtocolsConjugation2026-07-29T17:18:46ZGeneral Conjugation Protocol Return to BTK Page Conjugation is a reliable, robust method to transfer plasmids between bacteria. This is a general purpose protocol... (last changed by IraZibbu)IraZibbuProtocolListhttps://barricklab.org/twiki/bin/view/Lab/ProtocolList2026-05-27T21:56:24ZBarrick Lab :: Laboratory Protocols Updating Protocols How to Create a Protocol Best practices for designing a protocol and for putting it on the lab Wiki.... (last changed by JeffreyBarrick)JeffreyBarrickTestTopichttps://barricklab.org/twiki/bin/view/Lab/TestTopic2026-05-18T09:58:09Zasdasdf JeffreyBarrick 20 Jun 2009 (last changed by TestUser)TestUserWebHomehttps://barricklab.org/twiki/bin/view/Lab/WebHome2026-05-15T10:17:16Z.within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick)JeffreyBarrickReferencePlasmidshttps://barricklab.org/twiki/bin/view/Lab/ReferencePlasmids2026-05-13T15:26:23ZCommonly Used Plasmids plasmid markers origin host ref description pCA24N CamR Kitagawa2005 ASKA collection vector pKD... (last changed by IraZibbu)IraZibbuListofBioinformaticshttps://barricklab.org/twiki/bin/view/Lab/ListofBioinformatics2026-05-13T15:17:01ZBioinformatics Master List This page is a master list of bioinformatics software frequently used in the lab, and their purposes. It also includes additional notes... (last changed by IraZibbu)IraZibbuProtocolsBTKAssembleSingleTUPlasmidhttps://barricklab.org/twiki/bin/view/Lab/ProtocolsBTKAssembleSingleTUPlasmid2026-05-13T15:11:40ZBack to Golden Gate Protocols First Stage Plasmid (Transcriptional Unit) Assembly Once you have all your desired part plasmids built you can assemble them into Transcriptional... (last changed by AnthonyVandieren)AnthonyVandierenStrain_side_noteshttps://barricklab.org/twiki/bin/view/Lab/Strain_side_notes2026-05-13T15:01:32ZCommonly Used E. coli Strains OpenWetWare offers a comprehensive repository of the genotypes of the most commonly used E. coli strains. The following table points... (last changed by IraZibbu)IraZibbuLabPersonnelhttps://barricklab.org/twiki/bin/view/Lab/LabPersonnel2026-05-01T12:29:39ZBarrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick)JeffreyBarrickUTiGEMTeamhttps://barricklab.org/twiki/bin/view/Lab/UTiGEMTeam2026-04-30T10:09:33ZThe University of Texas at Austin :: iGEM Team The Barrick lab sponsored and mentored the UT Austin iGEM team from 2012 2025 when we were located at The University... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsElectrocompetentCellshttps://barricklab.org/twiki/bin/view/Lab/ProtocolsElectrocompetentCells2026-04-29T13:52:59ZElectrocompetent E. coli Making Electrocompetent E. coli Cells (small batch) This procedure makes enough electrocompetent cells for 2 3 transformations. 1 Grow... (last changed by IraZibbu)IraZibbuProtocols16SSequencinghttps://barricklab.org/twiki/bin/view/Lab/Protocols16SSequencing2026-04-28T19:00:14Z16S rRNA Sequencing to Identify Unknown Microbes Ever wonder what that contaminant in your culture is? Need to accurately identify an environmental isolate? Overview... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsRecipesABMShttps://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesABMS2026-04-28T00:45:40ZABMS: Acinetobacter Minimal Succinate Courtesy of the Averhoff lab, with modifications for available reagents. To make standard ABMS, combine the following pre sterilized... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsRecipesDavisMingiolihttps://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesDavisMingioli2026-04-22T18:39:45ZDM: Davis Mingioli Growth medium used by the long term E. coli evolution experiment. 0.5L 1L 4.5L 5L Component MW 2.67 g... (last changed by JeffreyBarrick)JeffreyBarrick
