TWiki's Lab webThe Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise.https://barricklab.org/twiki/bin/view/LabCopyright 2026 by contributing authors2026-07-06T23:35:32ZWebStatisticshttps://barricklab.org/twiki/bin/view/Lab/WebStatistics2026-07-06T23:35:32ZStatistics for Lab Web Month: Topic views: Topic saves: File uploads: Most popular topic views: Top contributors for topic save and... (last changed by TWikiGuest)TWikiGuestPublicationListhttps://barricklab.org/twiki/bin/view/Lab/PublicationList2026-06-13T05:42:42ZBarrick Lab :: Publications Barrick Lab Publications on Google Scholar Legend: Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick)JeffreyBarrickProtocolListhttps://barricklab.org/twiki/bin/view/Lab/ProtocolList2026-05-27T21:56:24ZBarrick Lab :: Laboratory Protocols Updating Protocols How to Create a Protocol Best practices for designing a protocol and for putting it on the lab Wiki.... (last changed by JeffreyBarrick)JeffreyBarrickTestTopichttps://barricklab.org/twiki/bin/view/Lab/TestTopic2026-05-18T09:58:09Zasdasdf JeffreyBarrick 20 Jun 2009 (last changed by TestUser)TestUserWebHomehttps://barricklab.org/twiki/bin/view/Lab/WebHome2026-05-15T10:17:16Z.within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick)JeffreyBarrickReferencePlasmidshttps://barricklab.org/twiki/bin/view/Lab/ReferencePlasmids2026-05-13T15:26:23ZCommonly Used Plasmids plasmid markers origin host ref description pCA24N CamR Kitagawa2005 ASKA collection vector pKD... (last changed by IraZibbu)IraZibbuListofBioinformaticshttps://barricklab.org/twiki/bin/view/Lab/ListofBioinformatics2026-05-13T15:17:01ZBioinformatics Master List This page is a master list of bioinformatics software frequently used in the lab, and their purposes. It also includes additional notes... (last changed by IraZibbu)IraZibbuProtocolsBTKAssembleSingleTUPlasmidhttps://barricklab.org/twiki/bin/view/Lab/ProtocolsBTKAssembleSingleTUPlasmid2026-05-13T15:11:40ZBack to Golden Gate Protocols First Stage Plasmid (Transcriptional Unit) Assembly Once you have all your desired part plasmids built you can assemble them into Transcriptional... (last changed by AnthonyVandieren)AnthonyVandierenStrain_side_noteshttps://barricklab.org/twiki/bin/view/Lab/Strain_side_notes2026-05-13T15:01:32ZCommonly Used E. coli Strains OpenWetWare offers a comprehensive repository of the genotypes of the most commonly used E. coli strains. The following table points... (last changed by IraZibbu)IraZibbuLabPersonnelhttps://barricklab.org/twiki/bin/view/Lab/LabPersonnel2026-05-01T12:29:39ZBarrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick)JeffreyBarrickUTiGEMTeamhttps://barricklab.org/twiki/bin/view/Lab/UTiGEMTeam2026-04-30T10:09:33ZThe University of Texas at Austin :: iGEM Team The Barrick lab sponsored and mentored the UT Austin iGEM team from 2012 2025 when we were located at The University... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsElectrocompetentCellshttps://barricklab.org/twiki/bin/view/Lab/ProtocolsElectrocompetentCells2026-04-29T13:52:59ZElectrocompetent E. coli Making Electrocompetent E. coli Cells (small batch) This procedure makes enough electrocompetent cells for 2 3 transformations. 1 Grow... (last changed by IraZibbu)IraZibbuProtocols16SSequencinghttps://barricklab.org/twiki/bin/view/Lab/Protocols16SSequencing2026-04-28T19:00:14Z16S rRNA Sequencing to Identify Unknown Microbes Ever wonder what that contaminant in your culture is? Need to accurately identify an environmental isolate? Overview... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsRecipesABMShttps://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesABMS2026-04-28T00:45:40ZABMS: Acinetobacter Minimal Succinate Courtesy of the Averhoff lab, with modifications for available reagents. To make standard ABMS, combine the following pre sterilized... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsRecipesDavisMingiolihttps://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesDavisMingioli2026-04-22T18:39:45ZDM: Davis Mingioli Growth medium used by the long term E. coli evolution experiment. 0.5L 1L 4.5L 5L Component MW 2.67 g... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsRecipesSOBhttps://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesSOB2026-04-22T17:15:10ZSOB/SOC: Super Optimal Broth For SOB: 200mL 250mL 1L Final Component 1 g 1.25 g 5 g 0.5% yeast extract... (last changed by JeffreyBarrick)JeffreyBarrick
TWiki's Lab webThe Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise.https://barricklab.org/twiki/bin/view/LabCopyright 2026 by contributing authors2026-07-06T23:35:32ZWebStatisticshttps://barricklab.org/twiki/bin/view/Lab/WebStatistics2026-07-06T23:35:32ZStatistics for Lab Web Month: Topic views: Topic saves: File uploads: Most popular topic views: Top contributors for topic save and... (last changed by TWikiGuest)TWikiGuestPublicationListhttps://barricklab.org/twiki/bin/view/Lab/PublicationList2026-06-13T05:42:42ZBarrick Lab :: Publications Barrick Lab Publications on Google Scholar Legend: Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick)JeffreyBarrickProtocolListhttps://barricklab.org/twiki/bin/view/Lab/ProtocolList2026-05-27T21:56:24ZBarrick Lab :: Laboratory Protocols Updating Protocols How to Create a Protocol Best practices for designing a protocol and for putting it on the lab Wiki.... (last changed by JeffreyBarrick)JeffreyBarrickTestTopichttps://barricklab.org/twiki/bin/view/Lab/TestTopic2026-05-18T09:58:09Zasdasdf JeffreyBarrick 20 Jun 2009 (last changed by TestUser)TestUserWebHomehttps://barricklab.org/twiki/bin/view/Lab/WebHome2026-05-15T10:17:16Z.within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick)JeffreyBarrickReferencePlasmidshttps://barricklab.org/twiki/bin/view/Lab/ReferencePlasmids2026-05-13T15:26:23ZCommonly Used Plasmids plasmid markers origin host ref description pCA24N CamR Kitagawa2005 ASKA collection vector pKD... (last changed by IraZibbu)IraZibbuListofBioinformaticshttps://barricklab.org/twiki/bin/view/Lab/ListofBioinformatics2026-05-13T15:17:01ZBioinformatics Master List This page is a master list of bioinformatics software frequently used in the lab, and their purposes. It also includes additional notes... (last changed by IraZibbu)IraZibbuProtocolsBTKAssembleSingleTUPlasmidhttps://barricklab.org/twiki/bin/view/Lab/ProtocolsBTKAssembleSingleTUPlasmid2026-05-13T15:11:40ZBack to Golden Gate Protocols First Stage Plasmid (Transcriptional Unit) Assembly Once you have all your desired part plasmids built you can assemble them into Transcriptional... (last changed by AnthonyVandieren)AnthonyVandierenStrain_side_noteshttps://barricklab.org/twiki/bin/view/Lab/Strain_side_notes2026-05-13T15:01:32ZCommonly Used E. coli Strains OpenWetWare offers a comprehensive repository of the genotypes of the most commonly used E. coli strains. The following table points... (last changed by IraZibbu)IraZibbuLabPersonnelhttps://barricklab.org/twiki/bin/view/Lab/LabPersonnel2026-05-01T12:29:39ZBarrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick)JeffreyBarrickUTiGEMTeamhttps://barricklab.org/twiki/bin/view/Lab/UTiGEMTeam2026-04-30T10:09:33ZThe University of Texas at Austin :: iGEM Team The Barrick lab sponsored and mentored the UT Austin iGEM team from 2012 2025 when we were located at The University... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsElectrocompetentCellshttps://barricklab.org/twiki/bin/view/Lab/ProtocolsElectrocompetentCells2026-04-29T13:52:59ZElectrocompetent E. coli Making Electrocompetent E. coli Cells (small batch) This procedure makes enough electrocompetent cells for 2 3 transformations. 1 Grow... (last changed by IraZibbu)IraZibbuProtocols16SSequencinghttps://barricklab.org/twiki/bin/view/Lab/Protocols16SSequencing2026-04-28T19:00:14Z16S rRNA Sequencing to Identify Unknown Microbes Ever wonder what that contaminant in your culture is? Need to accurately identify an environmental isolate? Overview... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsRecipesABMShttps://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesABMS2026-04-28T00:45:40ZABMS: Acinetobacter Minimal Succinate Courtesy of the Averhoff lab, with modifications for available reagents. To make standard ABMS, combine the following pre sterilized... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsRecipesDavisMingiolihttps://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesDavisMingioli2026-04-22T18:39:45ZDM: Davis Mingioli Growth medium used by the long term E. coli evolution experiment. 0.5L 1L 4.5L 5L Component MW 2.67 g... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsRecipesSOBhttps://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesSOB2026-04-22T17:15:10ZSOB/SOC: Super Optimal Broth For SOB: 200mL 250mL 1L Final Component 1 g 1.25 g 5 g 0.5% yeast extract... (last changed by JeffreyBarrick)JeffreyBarrick
