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| META TOPICPARENT |
name="ReferenceList" |
Commonly Used Plasmids |
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<
< |
- pCA24N (ASKA strain host)
- pKD3 (Cam template plasmids)
- pKD13 (Kan template plasmids)
- pCR2.1 (TOPO cloning kit vector)
- pACYC184 (Lenski evolution experiment) – tetR, cmR
- pKO3, pKOV (Church Lab vectors for creating deletions) – sacB, cmR
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>
> |
| plasmid |
markers |
description |
| pCA24N |
??? |
ASKA collection vector |
| pKD46 |
AmpR |
Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number. |
| pKD3 |
CamR |
Wanner template plasmid |
| pKD13 |
KanR |
Wanner template plasmid |
| pCR2.1 |
KanR, AmpR |
TOPO cloning kit vector |
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>
> |
| pACYC184 |
TetR, CamR |
Lenski evolution experiment |
| pKO3, pKOV |
SucS, CamR |
Church vectors for creating deletions |
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| META FILEATTACHMENT |
attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1" |
| META FILEATTACHMENT |
attachment="pCR2.1.gbk" attr="" comment="" date="1243603303" name="pCR2.1.gbk" path="pCR2.1.gbk" size="8323" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp42775" user="JeffreyBarrick" version="1" |
| META FILEATTACHMENT |
attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1" |
| META FILEATTACHMENT |
attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1" |
| META FILEATTACHMENT |
attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1" |
| META FILEATTACHMENT |
attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1" |
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