Difference: ReferencePlasmids (22 vs. 23)

Revision 232016-02-18 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	origin	host	ref	description
pCA24N	Cam^R			Kitagawa2005	ASKA collection vector
pKD46	Amp^R	TS	?	Datsenko&Wanner2000	Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD78	Cam^R	TS			Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3	Cam^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R				TOPO cloning kit vector
pACYC184	Tet^R, Cam^R		ER2420	Chang&Cohen1978	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R			Link1997	Church vectors for creating deletions; note: Grow at 30° Celsius
pACBSR	Cam^R	p15A		Herring2003	Low copy number. Use a midi or maxiprep.
pRA300, pRA301, pRA302	Spec^R			Akakura2002	Broad host range, promoterless lacZ map
pCP20	Amp^R	TS	BT340	FLP recombinase protocol Baba 2006	Temperature sensitive origin, FLP recombinase. Commonly used for removal of KAN cassette from Keio collection.

Added:

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pMMB67EH

Amp^R

RSF1010

Broad host range conjugative plasmid

Plasmids listed on this page reside in the common plasmid box in the –80°C freezers in both Welch and MBB labs.

Useful Links

Origins of replication and copy numbers of various plasmids and cosmids - Qiagen

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