Difference: ReferencePlasmids (20 vs. 21)

Revision 212014-02-11 - JeffreyBarrick

 
META TOPICPARENT name="ReferenceList"

Commonly Used Plasmids

plasmid markers origin host ref description
Changed:
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pCA24N CamR       Kitagawa2005 ASKA collection vector
>
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pCA24N CamR     Kitagawa2005 ASKA collection vector
 
pKD46 AmpR TS ? Datsenko&Wanner2000 Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD78 CamR TS     Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3 CamR   BW25141 Datsenko&Wanner2000 Wanner template plasmid
pKD13 KanR   BW25141 Datsenko&Wanner2000 Wanner template plasmid
pCR2.1 KanR, AmpR       TOPO cloning kit vector
pACYC184 TetR, CamR   ER2420 Chang&Cohen1978 Lenski evolution experiment
pKO3, pKOV SucS, CamR     Link1997 Church vectors for creating deletions; note: Grow at 30° Celsius
pACBSR CamR p15A   Herring2003 Low copy number. Use a midi or maxiprep.
pRA300, pRA301, pRA302 SpecR     Akakura2002 Broad host range, promoterless lacZ map
pCP20     BT340    

Plasmids listed on this page reside in the common plasmid box in the –80°C freezers in both Welch and MBB labs.

Useful Links

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META FILEATTACHMENT attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT attachment="pKD46.gb" attr="" comment="" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
META FILEATTACHMENT attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"
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