| |
| META TOPICPARENT |
name="ReferenceList" |
Commonly Used Plasmids
| plasmid |
markers |
origin |
ref |
description |
|
|
<
< |
| pCA24N |
CamR |
|
|
ASKA collection vector |
| pKD46 |
AmpR |
|
|
Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number. |
| pKD3 |
CamR |
|
|
Wanner template plasmid |
| pKD13 |
KanR |
|
|
Wanner template plasmid |
|
>
> |
|
| |
| pCR2.1 |
KanR, AmpR |
|
|
TOPO cloning kit vector |
|
|
<
< |
| pACYC184 |
TetR, CamR |
|
|
Lenski evolution experiment |
| pKO3, pKOV |
SucS, CamR |
|
|
Church vectors for creating deletions |
| pRA300, pRA301, pRA302 |
SpecR |
|
Akakura2002 |
Broad host range, promoterless lacZ map |
|
>
> |
| pACYC184 |
TetR, CamR |
|
Chang&Cohen1978 |
Lenski evolution experiment |
| pKO3, pKOV |
SucS, CamR |
|
Link1997 |
Church vectors for creating deletions |
| pRA300, pRA301, pRA302 |
SpecR |
|
Akakura2002 |
Broad host range, promoterless lacZ map |
|
| | |
|
>
> |
Plasmids currently in common plasmid box (located in -80 in both Welch and MBB)
| plasmid |
strain |
| pACBAR |
TOP10 |
| pKD3 |
BW25141 |
| pKD13 |
BW2514 |
| pKD32 |
BW2514 |
| pKD46 |
BW25141 |
| pCP20 |
BT340 |
| pRA300 |
? |
| pRA301 |
? |
| pRA302 |
? |
| pACYC184 |
ER2420 |
|
| | References
|
|
<
< |
| [Akakura2002] |
Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780. |
|
>
> |
| Akakura2002 |
Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780. |
|
|
>
> |
| Chang&Cohen1978 |
Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156. |
| Datsenko&Wanner2000 |
Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):6640–6645. |
| Kitagawa2005 |
Kitagawa, M. et al. (2005) DNA Res. 12: 291-9 |
| Link1997 |
Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37. |
|
| |
| META FILEATTACHMENT |
attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1" |
| META FILEATTACHMENT |
attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2" |
| META FILEATTACHMENT |
attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1" |
| META FILEATTACHMENT |
attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1" |
| META FILEATTACHMENT |
attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1" |
| META FILEATTACHMENT |
attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1" |
| META FILEATTACHMENT |
attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1" |
| META FILEATTACHMENT |
attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1" |
| META FILEATTACHMENT |
attachment="pKD46.gb" attr="" comment="lambda red plasmid genbank file" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1" |
| META FILEATTACHMENT |
attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1" |
|
