Installing breseq on stampede for mac

1. ssh into stampede and set up folder structures and modules:

• • ssh <username>@stampede.tacc.utexas.edu
  • mkdir local
  • mkdir $WORK/src
  • module load R
  • module load perl
  • module load bowtie/2.2.5
  • module load launcher
  • module save

1. Download the breseq repository from github and install it on stampede
   • cd $WORK/src
   • git clone https://github.com/barricklab/breseq.git
   • cd breseq
   • ./bootstrap.sh
   • ./configure --prefix=$HOME/local
   • make
   • make install
   • make test
2. Use the "find" function in the terminal window (command f) to look for the word "check" searching from the bottom of the window to the top of the window. You should see multiple instances of "passed check" and a few other random "checks" thrown in. Stop searching once you start seeing left aligned "checking" from the previous command. Assuming you do not see any messages of "failed check" you have successfully installed breseq.
3. Add breseq to your path
   • cdh
   • /usr/local/startup_scripts/install_default_scripts
   • nano .bashrc
     • in section 2 add the following line exactly as it is written to modify your path to include breseq's installation location then save and exit the text editor:
       • export PATH=$HOME/local/bin:$PATH
   • logout
   • ssh <username>@stampede.tacc.utexas.edu
   • which breseq

If all has gone well, you should get "~/local/bin/breseq" as a response. If you don't, troubleshoot.