| |
| META TOPICPARENT |
name="ProtocolsMediaRecipes" |
|
|
<
< | SOB: Super Optimal Broth |
>
> | SOB/SOC: Super Optimal Broth |
| | |
|
<
< | |
>
> | For SOB:
|
|
<
< | |
| |
| 1L |
Final [ ] |
Component |
| 5 g |
0.5% |
yeast extract |
| 20 g |
2.0% |
tryptone |
| 0.5 g |
10 mM |
NaCl |
| 0.186 g |
2 mM |
KCl |
|
|
<
< |
|
>
> |
| 2.4 g |
20 mM |
MgSO4 (anhydrous) |
|
|
>
> | Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L. Autoclave |
| | |
|
<
< | Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume of 960 ml. Autoclave. |
>
> | For SOC (=SOB+glucose):
|
|
>
> | Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% glucose.
Note: Some recipes for SOB use 10 mM MgSO4 and 10 mM MgCl. |
| | |
