Difference: ProtocolsRecipesSOB (1 vs. 2)

Revision 22013-09-03 - AlvaroRodriguez

 
META TOPICPARENT name="ProtocolsMediaRecipes"

SOB: Super Optimal Broth

1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
2.4 g 20 mM MgSO4
Changed:
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Add 800 ml of water and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add H2O to a final volume of 960 ml. Autoclave.
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Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume of 960 ml. Autoclave.
 

Revision 12012-01-11 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

SOB: Super Optimal Broth

1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
2.4 g 20 mM MgSO4

Add 800 ml of water and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add H2O to a final volume of 960 ml. Autoclave.

 
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