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| META TOPICPARENT |
name="ProtocolsMediaRecipes" |
MG: Minimal Glucose, aka DM: Davis Minimal |
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<
< | Note: Salts, agar and sugars need to be prepared and autoclaved separately |
>
> | Note: Salts, agar and sugars need to be prepared and autoclaved separately
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>
> | Also note: When making these plates it is necessary to prepare the 3 main parts (salts, agar, and sugar) separately . Each must be prepared and autoclaved independent of the others. Please follow the order below. |
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| 1L |
1.5L |
Component |
MW |
| 7 g |
10.5 g |
Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 |
MW 228.17 |
| 2 g |
3 g |
Potassium Phosphate (monobasic) H2KPO4 |
MW 136.07 |
| 1 g |
1.5 g |
Ammonium Sulfate (NH4)2 SO4 |
MW 132.08 |
| 0.5 g |
0.75 g |
Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 |
MW 294.10 |
| 333 ml |
500 ml |
dH2O |
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Autoclave.
Next, prepare the agar:
| 1L |
1.5L |
Component |
| 16 g |
24 g |
Agar |
| 1 ml |
1.5 ml |
Antifoam (5%) |
| 333 ml |
500 ml |
dH2O |
Autoclave.
Next, prepare the sugar (0.2%):
| 1L |
1.5L |
Component |
| 4 g |
6 g |
Glucose (or other sugar) |
| 333 ml |
500 ml |
dH2O |
Autoclave.
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<
< | After autoclaving combine the contents of the three flasks together and add the following stock solutions:
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>
> | After the 3 parts have been autoclaved, combine the contents of the three flasks together and add the following stock solutions:
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| 1L |
1.5L |
Component |
| 1.0 ml |
1.5 ml |
10% Magnesium Sulfate MgSO4 (autoclaved) |
| 1.0 ml |
1.5 ml |
0.2% Thiamin (vitamin B1) (filter sterilized) |
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