Add dH2O to 1 liter. Autoclave.
Combine the following ingredients (all pre-sterilized):
Added:
> >
1L
Component
200 ml
5× M9 salts
2 ml
1M Magnesium sulfate MgSO4
0.1 ml
1 M Calcium chloride CaCl2
40 ml
10% (w/v) glucose (or other carbon source)
Add sterile dH2O to 1 liter. Autoclave.
To make M9 plates add the items above to 16g of sterilized agar.
If making less than 1 liter use 8g of agar per 500ml of solution.
