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name="ProtocolsMediaRecipes" |
LB: Lysogeny Broth / Luria-Bertani (Miller) Agar
Combine in a 2 L flask the following:
| 1.5L |
Component |
| 15 g |
Tryptone |
| 7.5 g |
Yeast Extract |
| 15 g |
NaCl |
| 24 g |
Agar |
| 1.5 ml |
5% Antifoam |
Add dH2O to 1.5 L. Autoclave.
Notes and variations: |
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- This is the "Miller" formulation of LB. Another common formulation has only 5 g NaCl per liter! They are NOT interchangeable.
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- This is the "Miller" formulation of LB with 10 g NaCl per liter (1% w/v). Another common formulation has only 5 g NaCl per liter (0.5% w/v)! They are NOT interchangeable.
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- If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
- To make "soft" agar for phage studies, use only 7 g agar per liter.
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by="JeffreyBarrick" date="1435069038" from="Lab.ProtocolsRecipesLysogenyBroth" to="Lab.ProtocolsRecipesLysogenyBrothAgar" |
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