Difference:
ProtocolsRecipesDavisMingioli
(1 vs. 2)
Revision 2
2011-07-19 -
BrianRenda
META TOPICPARENT
name="ProtocolsMediaRecipes"
DM: Davis Minimal
7 g
Potassium Phosphate (dibasic, trihydrate) HK
2
PO
4
(H
2
O)
3
MW 228.17
Changed:
<
<
5 g
Potassium Phosphate (monobasic) H
2
KPO
4
MW 136.07
>
>
2 g
Potassium Phosphate (monobasic) H
2
KPO
4
MW 136.07
1 g
Ammonium Sulfate (NH
4
)
2
SO
4
MW 132.08
0.5 g
Sodium Citrate (trisodium, dihydrate) Na
3
C
6
H
5
O
7
(H
2
O)
2
MW 294.10
Add dH
2
O to 1 liter. Autoclave. Then add
1 ml
10% Magnesium Sulfate MgSO
4
MW 120.37
Note* The MgSO
4
must be autoclaved separately.
1 ml
0.2% Thiamin (vitamin B1)
Final nutrient concentrations:
67.4 mM
Phosphate (PO
4
3-
)
8.38 mM
Sulfate (SO
4
2-
)
15.1 mM
Ammonium (NH
4
)
5.1 mM
Sodium (Na
+
)
98.1 mM
Potassium (K
+
)
0.83 mM
Magnesium (MGgsup>2+)
1.7 mM
Citrate
139 µM
Glucose (in DM25)
Revision 1
2011-06-14 -
CraigBarnhart
META TOPICPARENT
name="ProtocolsMediaRecipes"
DM: Davis Minimal
7 g
Potassium Phosphate (dibasic, trihydrate) HK
2
PO
4
(H
2
O)
3
MW 228.17
5 g
Potassium Phosphate (monobasic) H
2
KPO
4
MW 136.07
1 g
Ammonium Sulfate (NH
4
)
2
SO
4
MW 132.08
0.5 g
Sodium Citrate (trisodium, dihydrate) Na
3
C
6
H
5
O
7
(H
2
O)
2
MW 294.10
Add dH
2
O to 1 liter. Autoclave. Then add
1 ml
10% Magnesium Sulfate MgSO
4
MW 120.37
Note* The MgSO
4
must be autoclaved separately.
1 ml
0.2% Thiamin (vitamin B1)
Final nutrient concentrations:
67.4 mM
Phosphate (PO
4
3-
)
8.38 mM
Sulfate (SO
4
2-
)
15.1 mM
Ammonium (NH
4
)
5.1 mM
Sodium (Na
+
)
98.1 mM
Potassium (K
+
)
0.83 mM
Magnesium (MGgsup>2+)
1.7 mM
Citrate
139 µM
Glucose (in DM25)
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