Sterile Saline

Purpose: Used make dilutions of viable cells for plating or transfer to new media.

This saline concentration of 0.85% w/v (145 mM) is suitable for diluting E. coli before transferring to new media or plating on agar.

1L 5L Component
8.5 g 42.5 g NaCl

Add dH2O to final volume. Autoclave before use. Prepare large batches in 6 L flask and then aliquot out into bottles. It is not important to measure out exact volumes when dividing a large batch among bottles.

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Topic revision: r4 - 03 Sep 2013 - 03:44:19 - Main.AlvaroRodriguez
 
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