SOB/SOC: Super Optimal Broth

For SOB:

1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
2.4 g 20 mM MgSO4 (anhydrous)
Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L. Autoclave

For SOC (=SOB+glucose):
Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% (w/v) glucose. (That's a final concentration of 0.4% glucose.)

Note: Some recipes for SOB use 10 mM MgSO4 and 10 mM MgCl.

 Barrick Lab  >  ProtocolsMediaRecipes  >  ProtocolsRecipesSOB

Topic revision: r4 - 23 Dec 2018 - 18:47:11 - Main.JeffreyBarrick
 
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