LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

Combine in a 2 L flask the following:

1.5L Component
15 g Tryptone
7.5 g Yeast Extract
15 g NaCl
24 g Agar
1.5 ml 5% Antifoam

Add dH2O to 1.5 L. Autoclave.

Notes and variations:

  • Common synonyms are Luria-Broth and Luria-Bertani medium.
  • This is the "Miller" formulation of LB. Another common formulation has only 5 g NaCl per liter! They are NOT interchangeable.
  • If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
  • To make "soft" agar for phage studies, use only 7 g agar per liter.

 Barrick Lab  >  ProtocolsMediaRecipes  >  ProtocolsRecipesLysogenyBrothAgar

Topic revision: r6 - 23 Jun 2015 - 14:20:34 - Main.JeffreyBarrick
Lab.ProtocolsRecipesLysogenyBrothAgar moved from Lab.ProtocolsRecipesLysogenyBroth on 23 Jun 2015 - 14:17 by Main.JeffreyBarrick - put it back
 
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