Working with Arsenophonus nasoniae

Arsenophonus nasoniae is symbiont of the wasp, Nasonia vitripennis, gut microbiota that can be cultivated in vitro. The isolation and characterization of the particular strain (15247) used was carried out by Dr. Robert L. Gherna and are described in depth in here.

A second A. nasoniae strain (24899) was isolated from the tick, Ixodes ricinus, by Oleg Mediannikov et. al and is described here.

In vitro culture conditions

Plate Growth

Arsenophonus nasoniae DSMZ Strain: [[https://www.dsmz.de/catalogues/details/culture/DSM-15247.html?tx_dsmzresources_pi5[returnPid]=304][15247]] can be cultured at room temperature; although recommended culturing temperature found in literature is 28C. It grows robustly on agar plates after 3-5 days on the following media:
  • Brain Heart Infusion Agar

This particular strain doesn't show any antibiotic resistance to antibiotics at E. coli concentrations, so all standard lab concentrations can be used when making plates.

Arsenophonus nasoniae DSMZ Strain: 24899 can be cultured at room temperature; although recommended culturing temperature found in literature is 28C. It grows robustly on agar plates after >1 week on the following media:

  • Colombia Blood Agar

Liquid Media

Arsenophonus nasoniae 15247 can be cultivated in the following liquid media (same general culture conditions as above, with shaking):

  • Brain Heart Infusion Media

Strain 24899 can be cultivated in the following liquid media (same general culture conditions as above, with shaking):

  • Brain Heart Infusion Media
  • Trypticase Soy Broth

-- Main.KateElston - 23 Feb 2017

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Topic revision: r2 - 25 May 2017 - 19:36:44 - Main.KateElston
 
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