Pulsed Field Gel Electrophoresis Mapping
Purpose: To validate mutations predicted from whole-genome re-sequencing and possibly discover rearrangements through repetitive sequences or tandem duplications that are difficult to predict from sequencing data.
Preparation of Plugs
Digestion with Restriction Enzyme
Running Pulsed Field Gel
Analysis of Gel
Topic revision: r1 - 2009-08-10 - 18:39:52 - Main.JeffreyBarrick