(r3) Barrick Lab :: ProceduresChangingEnvironmentLongTerm

Contamination check. Set up plates with a checkerboard pattern of Ara+ and Ara- marked cells. On odd-numbered plates an Ara- is in well A1. On even-numbered plates an Ara+ strain is in well A1.

Daily, transfer 3.5 µl into 900 µl of fresh DM0 + 0.01% carbon compound.

At regular intervals, take a plate after it has

Plate 1

Red Plate. Strains 606/607. 8 Replicates, one per column.

1	1	D-Glucose
2	2	Glycerol
3	3	β-Lactose
4	4	D-Fructose
5	5	D-Ribose
6	6	D-Galactose
7	7	L-Rhamnose
8	8	Melibiose
9	9	D-Gluconic Acid
10	10	D-Sorbitol
11	11	Acetate
12	12	L-Malic Acid

Plate 2

White Plate. Strains 606/607. 8 Replicates, one per column.

1	13	Pyruvic Acid
2	14	DL-Lactic Acid
3	17	Thymidine
4	18	Inosine
5	19	D-Trehalose
6	20	D-Serine
7	21	D-Glucuronic Acid
8	22	L-Alanine
9	23	D-Glucosamine
10	37	Glucuronamide
11	71	D-Arabinose
12	72	L-Proline

Plate 3

Red Plate. Strains 606/607. 8 Replicates, one per column.

	Treatment	Days
1	L-Proline, Thymidine, Acetate, D-Glucose	1
2	L-Proline, Thymidine, Acetate, D-Glucose	2
3	L-Proline, Thymidine, Acetate, D-Glucose	4
4	L-Proline, Thymidine, Acetate, D-Glucose	8
5	L-Proline, Thymidine, Acetate, D-Glucose	16
6	L-Proline, Thymidine, Acetate, D-Glucose	32
7	Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose	1
8	Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose	2
9	Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose	4
10	Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose	8
11	Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose	16
12	Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose	32

Plate 4

White Plate. Strains 606/607. 8 Replicates, one per column.

	Treatment	Days
1	L-Rhamnose, D-Galactose, D-Fructose, D-Glucose	1
2	L-Rhamnose, D-Galactose, D-Fructose, D-Glucose	2
3	L-Rhamnose, D-Galactose, D-Fructose, D-Glucose	4
4	L-Rhamnose, D-Galactose, D-Fructose, D-Glucose	8
5	L-Rhamnose, D-Galactose, D-Fructose, D-Glucose	16
6	L-Rhamnose, D-Galactose, D-Fructose, D-Glucose	32
7	Acetate, D-Ribose, Glycerol, D-Glucose	1
8	Acetate, D-Ribose, Glycerol, D-Glucose	2
9	Acetate, D-Ribose, Glycerol, D-Glucose	4
10	Acetate, D-Ribose, Glycerol, D-Glucose	8
11	Acetate, D-Ribose, Glycerol, D-Glucose	16
12	Acetate, D-Ribose, Glycerol, D-Glucose	32

Plate 5

Red Plate. Strains 606/607. 8 Replicates, one per column.

	Treatment	Days
1	Random 1	1
2	Random 2	1
3	Random 3	1
4	Random 4	1
5	Random 5	1
6	Random 6	1
7	Random 7	1
8	Random 8	1
9	Random 9	1
10	Random 10	1
11	Random 11	1
12	Random 12	1

-- Main.JeffreyBarrick - 24 Sep 2007

Barrick Lab > ProceduresChangingEnvironmentLongTerm

Contributors to this topic

JeffreyBarrick, MarkKauth

Topic revision: r3 - 2007-09-24 - 17:17:39 - Main.JeffreyBarrick