Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	683,496	A→C	V65G (GTT→GGT)	nagC	DNA‑binding transcriptional dual regulator, repressor of N‑acetylglucosamine

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	683,496	0	A→C	100.0%	194.8	60	V65G (GTT→GGT)	nagC	DNA‑binding transcriptional dual regulator, repressor of N‑acetylglucosamine
Reads supporting (aligned to +/- strand):  new base (24/36):  ref base (0/0):  total (24/36)

GCGCGGCGGCCCCCAGTGGAGGCCTGCTGATCAACCTCTTTGATCAGCCCGCGTTCGATAAGCTGA  >  REL606/683463‑683528                                  |                                 gcgcGGCGGCCCCCAGTGGAGGCCTGCTGATCACcc                                <  1:14596/36‑1 (MQ=255)  cgcgGCGGCCCCCAGTGGAGGCCTGCTGATCACCct                               <  1:3601440/36‑1 (MQ=255)  cgcgGCGGCCCCCAGTGGAGGCCTGCTGATCACCct                               <  1:6727403/36‑1 (MQ=255)  cgcgGCGGCCCCCAGTGGAGGCCTGCTGATCACCct                               <  1:3166465/36‑1 (MQ=255)  cgcgGCGGCCCCCAGTGGAGGCCTGCTGATCACCct                               <  1:5298743/36‑1 (MQ=255)   gcggcgGCCCCCAGTGGAGGCCTGCTGATCACCctc                              <  1:5477864/36‑1 (MQ=255)   gcggccGCCCCCAGTGGAGGCCTGCTGATCACCctc                              <  1:4646999/36‑1 (MQ=25)    cggcggCCCCCAGTGGAGGCCTGCTGATCACCctct                             <  1:5742518/36‑1 (MQ=255)    cggcggCCCCCAGTGGAGGCCTGCTGATCACCctct                             >  1:5676174/1‑36 (MQ=255)     ggcggcCCCCAGTGGAGGCCTGCTGATCACCCTCtt                            <  1:2292446/36‑1 (MQ=255)       cggcCCCCAGTGGAGGCCTGCTGATCACCCTCTTTg                          >  1:6644795/1‑36 (MQ=255)       ccgcCCCCAGTGGAGGCCTGCTGATCACCCTCTTTg                          <  1:6655776/34‑1 (MQ=25)        ggcCCCCAGTGGAGGCCTGCTGATCACCCTCTTTGa                         <  1:6580951/36‑1 (MQ=255)        ggcCCCCAGTGGAGGCCTGCTGATCACCCTCTTTGa                         <  1:5640709/36‑1 (MQ=255)        ggcCCCCAGTGGAGGCCTGCTGAGCACCCTCTTTGa                         <  1:5876441/36‑1 (MQ=25)          cccccAGTGGAGGCCTGCTGATCACCCTCTTTGATc                       <  1:6859015/36‑1 (MQ=255)          cccccAGTGGAGGCCTGCTGATCACCCTCTTTGATc                       <  1:3163302/36‑1 (MQ=255)           ccccGGTGGAGGCCTGCTGATCACCCTCTTTGATCa                      <  1:4588575/36‑1 (MQ=25)           ccccAGTGGAGGCCTGCTGATCACCCTCTTTGATCa                      <  1:6147572/36‑1 (MQ=255)           ccccAGTGGAGGCCTGCTGATCACCCTCTTTGATCa                      <  1:1690733/36‑1 (MQ=255)            cccAGTGGAGGCCTGCTGATCACCCTCTTTGATCAg                     >  1:322723/1‑36 (MQ=255)            cccAGGGGAGGCCTGCTGATCACCCTCTGGGATCAg                     <  1:4795073/36‑1 (MQ=18)              cggTGGAGGCCTGCTGATCACCCTCTTTGATCAGcc                   <  1:2410003/34‑1 (MQ=25)              cAGTGGAGGCCTGCTGATCACCCTCTTTGATCAGcc                   >  1:2333921/1‑36 (MQ=255)              cAGTGGAGGCCTGCTGATCACCCTCTTTGATCAGcc                   >  1:2160074/1‑36 (MQ=255)              cAGGGGAGGCCTGCTGATCACCCTCTTTGATCAGcc                   <  1:4870768/36‑1 (MQ=25)               aGTGGAGGCCTGCTGATCACCCTCTTTGATCAGccc                  >  1:1814721/1‑36 (MQ=255)               aGTGGAGGCCTGCTGATCACCCTCTTTGATCAGccc                  <  1:6248248/36‑1 (MQ=255)               aGTGGAGGCCTGCTCATCACCCTCTTTTATCAAccc                  >  1:2412454/1‑36 (MQ=18)                gTGGAGGCCTGCTGATCACCCTCTTTGATCAGCCcg                 <  1:1754453/36‑1 (MQ=255)                gTGGAGGACTGCTGATCACCCTCTTTGATCAGCCcg                 >  1:208724/1‑36 (MQ=25)                 tGGAGGCCTGCTGATCACCCTCTTTGATCAGCCcgc                >  1:3505569/1‑36 (MQ=255)                  ggAGGCCTGCTGATCACCCTCTTTGATCAGCCcgcg               >  1:4745169/1‑36 (MQ=255)                   gAGGCCTGCTGATCACCCTCTTTGAGCAGCCCGCGt              <  1:5597434/36‑1 (MQ=25)                    agcccTGCTGATCACCCTCTTTGATCAGCCCGCGtt             <  1:7206759/33‑1 (MQ=25)                    aGGGCTGCTGATCACCCTCTTTGATCAGCCCGCGtt             <  1:5720218/36‑1 (MQ=25)                      gCCTGCTGATCACCCTCTTTGATCAGCCCGCGTTCg           <  1:6483497/36‑1 (MQ=255)                      gCCTGCTGATCACCCTCTTTGATCAGCCCGCGTTCg           <  1:3319300/36‑1 (MQ=255)                        ctgctgGTCACCGTCTGTGATCAGCCCGCGTTCGAt         <  1:95532/36‑1 (MQ=18)                        ctgctgATCACCCTCTTTGATCAGCCCGCGTTCGAt         >  1:5736530/1‑36 (MQ=255)                        ctgctgATCACCCTCTTTGATCAGCCCGCGTTCGAt         <  1:2049290/36‑1 (MQ=255)                        ctgctgATCACCCTCTTTGATCAGCCCGCGTTCGAt         <  1:4983649/36‑1 (MQ=255)                         tgctgGTCACCCTCTTTGATCAGCCCGCGTTCGATa        <  1:5636239/36‑1 (MQ=25)                         tgctgATCACCCTCTTTGATCAGCCCGCGTTCGATa        >  1:6155337/1‑36 (MQ=255)                         tgctgATCACCCTCTTTGATCAGCCCGCGTTCGATa        >  1:989125/1‑36 (MQ=255)                         tgctgATCACCCTCTTTGATCAGCCCGCGTTCGATa        >  1:5797172/1‑36 (MQ=255)                         tgctgATCACCCTCTTTGATCAGCCCGCGTTCGATa        >  1:2964142/1‑36 (MQ=255)                           ctgATCACCCTCTTTAATCAGCCCGCGTTCGATAAg      >  1:1616952/1‑36 (MQ=25)                            tgATCACCCTCTTTGATCGGCCCGCGTTCGATAAGc     <  1:1096693/36‑1 (MQ=25)                            tgATCACCCTCTTTGATCAGCCCGCGTTCGATAAGc     >  1:5809441/1‑36 (MQ=255)                             gATCACGCTCTTTGATCAGCCCGCGTTCGATAAGCt    <  1:622419/36‑1 (MQ=25)                             gATCACCCTCTTTGATCAGCCCGCGTTTGATAAGCt    >  1:3141840/1‑36 (MQ=25)                             gATCACCCTCTTTGATCAGCCCGCGTTCGATATGCt    >  1:4083917/1‑36 (MQ=25)                             gATCACCCTCTTTGATCAGCCCGCGTTCGATAAGCt    >  1:5381037/1‑36 (MQ=255)                             gATCACCCTCTTTGATCAGCCCGCGTTCGATAAGCt    >  1:476776/1‑36 (MQ=255)                             gATCACCCTCTTTGATCAGCCCGCGTTCGAAAAGCt    >  1:6515816/1‑36 (MQ=25)                              aTCACCCTCTTTGATCAGCCCGCGTTCGATAAGCTg   >  1:5890251/1‑36 (MQ=255)                              aTCACCCTCTTTGATCAGCCCGCGTTCGATAAGCTg   <  1:5789558/36‑1 (MQ=255)                              aTCACCCTCTTTGATCAGCCCGCGTTCGATAAGCTg   <  1:4059865/36‑1 (MQ=255)                               tCACCCTCTTTGATCAGCCCGCGTTCGTTAAGCTGa  >  1:6952739/1‑36 (MQ=25)                                  |                                 GCGCGGCGGCCCCCAGTGGAGGCCTGCTGATCAACCTCTTTGATCAGCCCGCGTTCGATAAGCTGA  >  REL606/683463‑683528 Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 32 ≤  ATCG