Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	668,787	A→C	Y65D (TAC→GAC)	gltI	glutamate and aspartate transporter subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	668,787	0	A→C	100.0%	138.5	42	Y65D (TAC→GAC)	gltI	glutamate and aspartate transporter subunit
Reads supporting (aligned to +/- strand):  new base (19/23):  ref base (0/0):  total (19/23)

CTTCAACAATGGCGTTGGAGTAATCCTGCGAGTAACCCACCACTTTTTGCTGATTGTCGTAATAA  >  REL606/668754‑668818                                  |                                cTTCAACAATGGCGTTGGAGTAATCCTGCGAGTCAc                               <  1:775578/36‑1 (MQ=255)   tCAACAATGGCGTTGGAGTAATCCTGCGAGTCAccc                             >  1:1879570/1‑36 (MQ=255)   tCAACAATGGCGTTGGAGTAATCCTGCGAGTCAccc                             <  1:3049263/36‑1 (MQ=255)     aacaaTGGCGTTGGAGTAATCCTGCGAGTCACccac                           >  1:5195051/1‑36 (MQ=255)     aacaaTGGCGTTGGAGTAATCCTGCGAGTCACcaac                           <  1:1856536/36‑1 (MQ=255)      acaaTGGCGTTGGAGTAATCCTGCGAGTCAAccacc                          >  1:6593756/1‑36 (MQ=255)       cgaTGGGGTGGGAGTAATCCTGCGAGTCACccacca                         <  1:1854764/34‑1 (MQ=21)        aaTGGCGTTGGAGTAATCCTGCGAGTCACCcaccac                        >  1:3700981/1‑36 (MQ=255)         aTGGCGTTGGAGTAATCCTGCGAGTCACCCACCACt                       >  1:849476/1‑36 (MQ=255)            gCGTTGGAGTAATCCTGCGAGTCACCCACCACtttt                    <  1:3041586/36‑1 (MQ=255)            gCGTTGAAGTAATCCTGCGAGTCACCCACCACtttt                    <  1:1049264/36‑1 (MQ=25)             cGTTGGAGTAATCCTGCGAGTCACCCACCACttttt                   <  1:1288079/36‑1 (MQ=255)             cGTTGGAGTAATCCTGCGAGTCACCCACCACttttt                   >  1:3859145/1‑36 (MQ=255)             cGTTGGAGTAATCCTGCGAGTCACCCACCACttttt                   >  1:7428607/1‑36 (MQ=255)              gTTGGGGTAATCCTGCGAGTCACCCACCACTTTTTg                  <  1:4823905/36‑1 (MQ=25)               ttGGAGTAATCCGGCGAGTCACCCACCACTTTTTGc                 <  1:2591375/36‑1 (MQ=25)                tGGAGTAATCCTGCGAGTCACCCACCACTTTTTGCt                <  1:239504/36‑1 (MQ=255)                tGGAGTAATCCTGCGAGTCACCCACCACTTTTTGCt                <  1:857985/36‑1 (MQ=255)                 ggAGTAATCCTGCGAGTCACCCACCACTTTTTGCTg               <  1:6643338/36‑1 (MQ=255)                 ggAGTAATCCTGCGAGTCACCCACCACTTTTTGCTg               >  1:2113189/1‑36 (MQ=255)                   aGTAATCCTGCGAGTCACCCACCACTTTTTGCTGAt             <  1:1267625/36‑1 (MQ=255)                    gTAATCCTGCGAGTCACCCACCACTTTTTGCTGAtt            >  1:951642/1‑36 (MQ=255)                    gTAATCCAGCGAGTCACCCACCACTTTTTGCTGAtt            <  1:3350589/36‑1 (MQ=21)                    gTAAGCGTGCGAGTCACCCACCACTTTTTGCTGAtt            <  1:5783627/36‑1 (MQ=18)                     tAATCCTGCGAGTCACCCACCACTTTTTGCTGATTg           >  1:6702397/1‑36 (MQ=255)                     tAATCCTGCGAGTCACCCACCACTTTTTGCTGATTg           >  1:4660717/1‑36 (MQ=255)                      aaTCCTGCGAGTCACCCACCACTTTTTGCTGATTGt          >  1:7439820/1‑36 (MQ=255)                      aaTCCTGCGAGTCACCCACCACTTTTTGCTGATTGt          >  1:3439492/1‑36 (MQ=255)                       acccTGCGAGTCACCCACCACTTTTTGCTGATTGTc         <  1:986382/34‑1 (MQ=21)                       aTCCTGCGAGTCACCCACCACTTTTTGCTGATTGTc         <  1:3651837/36‑1 (MQ=255)                         ccTGCGAGTCACCCACCACTTTTTGCTGATTGTCGt       >  1:6838817/1‑36 (MQ=255)                         ccTGCGAGTCACCCACCACTTTTTGCTGATTGTCGt       <  1:2369268/36‑1 (MQ=255)                          cTGCGAGTCACCCACCCCCTCTTGCTGATTGTCGta      >  1:3584806/1‑36 (MQ=18)                          cTGCGAGTCACCCACCACTTTTTGCTGATTGTCGta      >  1:6283017/1‑36 (MQ=255)                          cTGCGAGTCACCCACCACTTTTTGCTGATTGTCGta      <  1:2011156/36‑1 (MQ=255)                           tGCGAGTCACCCACCACTTTTTGCTGATTGTCGtat     >  1:2200615/1‑35 (MQ=255)                           tGCGAGTCACCCACCACTCTTTGCTGATTGTCGtaa     >  1:7383697/1‑36 (MQ=25)                            gCGAGTCACCCACCACTTTTTGCTGATTGTCGtaat    <  1:7481599/36‑1 (MQ=255)                             cGAGTCACCCACCACTTTTTGCTGATTGTCGtaata   <  1:937446/36‑1 (MQ=255)                             cGAGTCACCCACCACTTTTTGCTGATTGTCGtaata   <  1:5780404/36‑1 (MQ=255)                              gAGTCACCCACCACTTTTTGCTGATTGTCGtaataa  >  1:7132719/1‑36 (MQ=255)                              gAGTCACCCACCACTTTTTGCTGATTGTCGtaataa  <  1:1868567/36‑1 (MQ=255)                                  |                                CTTCAACAATGGCGTTGGAGTAATCCTGCGAGTAACCCACCACTTTTTGCTGATTGTCGTAATAA  >  REL606/668754‑668818 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 32 ≤  ATCG