Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,370,027	T→A	K117M (AAG→ATG)	rpsM	30S ribosomal protein S13

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	3,370,027	0	T→A	100.0%	183.0	56	K117M (AAG→ATG)	rpsM	30S ribosomal protein S13
Reads supporting (aligned to +/- strand):  new base (22/34):  ref base (0/0):  total (22/34)

CCTTTGCCATTATTCAATCACCCCGATTATTTCTTGATCGGTTTGCGCGGACCCTTACGGGTACGT  >  REL606/3369994‑3370059                                  |                                 cgtttGGCATTATTCAATCACCCCGATTATTTCATg                                <  1:1791763/34‑1 (MQ=21) ccTTTGCCATTATTCAATCACCCCGATTATTTCATg                                <  1:5971304/36‑1 (MQ=255) ccTTTGCCATTATTCAATCACCCCGATTATTTCATg                                <  1:2734448/36‑1 (MQ=255)  cTTTGCCATTATTCAATCACCCCGATTATTTCATGa                               >  1:2991536/1‑36 (MQ=255)  cTTTGCCATTATTCAATCACCCCGATTATTTCATGa                               <  1:1721314/36‑1 (MQ=255)  cTTTGCCATTATTCAATCACCCCGATTATTTCATGa                               >  1:5491102/1‑36 (MQ=255)     tGCCATTATTCAATCACCCCGATTATTTCATGATca                            <  1:2827482/36‑2 (MQ=255)     tGCCATTATTCAATCACCCCGATTATTTCATGATCg                            >  1:3354466/1‑36 (MQ=255)     tGCCATTATTCAATCACCCCGATTATTTCATGATCg                            <  1:6751946/36‑1 (MQ=255)      gCCATTATTCAATCACCCCGATTATTTCATGATCgg                           >  1:2688297/1‑36 (MQ=255)      gCCATTATTCAATCACCCCGAATATTTCATGATCgg                           <  1:6370591/36‑1 (MQ=25)       ccATTATTCAATCACCCCGATTATTTCATGATCGGt                          <  1:4203817/36‑1 (MQ=255)       ccATTATTCAATCACCCCGATTATTTCATGATCGGt                          <  1:16553/36‑1 (MQ=255)       ccATTATTCAATCACCCCGATTATTTCATGATCGGt                          >  1:6572770/1‑36 (MQ=255)       ccATTATTCAATCACCCCGATTATTTCATGATCGGt                          >  1:3311939/1‑36 (MQ=255)       ccAGTATTCAATCACCCCGATTATTTCATGATCGGt                          <  1:5354117/36‑1 (MQ=25)        cATTATTCAATCACCCCGATTATTTCATGATCGGtt                         >  1:5646479/1‑36 (MQ=255)        cATTATTCAATCACCCCGATTATTTCATGATCGGtt                         >  1:3112723/1‑36 (MQ=255)         attattCAATCACCCCGATTATTTCATGATCGGttt                        >  1:427871/1‑36 (MQ=255)         attattCAATCACCCCGATTATTTCATGATCGGttt                        >  1:3732391/1‑36 (MQ=255)          ttattCAATCACCCCGATTATTTCATGATCGGTTTg                       <  1:1043146/36‑1 (MQ=255)            attCAATCACCCCGATTATTTCATGATCGGTTTgcg                     <  1:677851/36‑1 (MQ=255)            attCAATCACCCCGATTATTTCATGATCGGTTTgcg                     <  1:3915009/36‑1 (MQ=255)             ttCAATCACCCCGATTATTTCATGATCGGTTTgcgc                    >  1:6987200/1‑36 (MQ=255)             ttCAATCACCCCGATTATTTCATGATCGGTTTgcgc                    <  1:698054/36‑1 (MQ=255)             ttCAATCACCCCGATTATTTCATGATCGGTTTgcgc                    <  1:2074755/36‑1 (MQ=255)             ttCAATCACCCCGATTATTTCATGATCGGTTTgcgc                    <  1:4909630/36‑1 (MQ=255)               cAATCACCCCGATTATTTCATGATCGGTTTGCGCgg                  >  1:5423279/1‑36 (MQ=255)               cAATCACCCCGATTATTTCATGATCGGTTTGCGCgg                  <  1:5282242/36‑1 (MQ=255)               cAATCACCCCGATTATTTCATGATCGGTTTGCGCgg                  <  1:5033765/36‑1 (MQ=255)               cAATCACCCCGATTATTTCATGATCGGTTTGCGCgg                  <  1:1046515/36‑1 (MQ=255)               cAATCACCCCGATTATTTCATGATCGGTTTGCGCgg                  >  1:702598/1‑36 (MQ=255)                 aTCACCCCGATTATTTCATGATCGGTTTGCGCGGAc                <  1:5877403/36‑1 (MQ=255)                   cACCCCGATTATTTCATGATCGGTTTGCGCGGAccc              <  1:6338287/36‑1 (MQ=255)                   cACCCCGATTATTTCATGATCGGTTTGCGCGGAccc              >  1:2455065/1‑36 (MQ=255)                    aCCCCGATTATTTCATGATCGGTTTGCGCGGACCCt             <  1:5712726/36‑1 (MQ=255)                    aCCCCGATTATTTCATGATCGGTTTGCGCGGACCCt             <  1:5388309/36‑1 (MQ=255)                     ccccGATTATTTCATGATCGGTTTGCGCGGACCCtt            >  1:2401287/1‑36 (MQ=255)                     ccccGATTATTTCATGATCGGTTTGCGCGGACCCtt            >  1:1079620/1‑36 (MQ=255)                       ccGATTATTTCATGATCGGTTTGCGCGGACCCTTAc          <  1:3388816/36‑1 (MQ=255)                        cGATTATTTCATGATCGGTTTGCGCGGACCCTTACg         <  1:26093/36‑1 (MQ=255)                        cGATTATTTCATGATCGGTTTGCGCGGACCCTTACg         <  1:4479709/36‑1 (MQ=255)                         gATTATTTCATGATCGGTTTGCGCGGACCCTTACgg        >  1:7357691/1‑36 (MQ=255)                         gATTATTTCATGATCGGTTTGCGCGGACCCTTACgg        <  1:4141598/36‑1 (MQ=255)                         gATTATTTCATGATCGGTTTGCGCGGACCCTTACgg        <  1:2319218/36‑1 (MQ=255)                          attattTCATGATCGGTTTGCGCGGACCCTTACggg       >  1:4197322/1‑36 (MQ=255)                          attattTCATGATCGGTTTGCGCGGACCCTTACggg       >  1:5019682/1‑36 (MQ=255)                          attattTCATGATCGGTTTGCGCGGACCCTTACggg       <  1:5509990/36‑1 (MQ=255)                            ttattCATGATCGGTTTGCGCGGACCCTTACGGGTa     <  1:1930949/33‑1 (MQ=25)                            tattTCATGATCGGTTTGCGCGGACCCTTACGGGTa     <  1:1106239/36‑1 (MQ=255)                            tattTCATGATCGGTTTGCGCGGACCCTTACGGGTa     >  1:4685447/1‑36 (MQ=255)                             attTCATGATCGGTTTGCGCGGACCCTTACGGGTAc    <  1:6694610/36‑1 (MQ=255)                             attTCATGATCGGTTTGCGCGGACCCTTACGGGTAc    <  1:3408130/36‑1 (MQ=255)                              tttCATGATCGGTTTGCGCGGACCCTTACGGGTACg   <  1:3593969/36‑1 (MQ=255)                               ttCATGATCGGTTTGCGCGGACCCTTACGGGTACGt  >  1:6217986/1‑36 (MQ=255)                               ttCATGATCGGTTTGCGCGGACCCTTACGGGTACGt  >  1:3389483/1‑36 (MQ=255)                                  |                                 CCTTTGCCATTATTCAATCACCCCGATTATTTCTTGATCGGTTTGCGCGGACCCTTACGGGTACGT  >  REL606/3369994‑3370059 Base quality scores:  ATCG  < 3 ≤  ATCG  < 14 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 32 ≤  ATCG