Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,339,158	A→C	intergenic (+22/‑4)	yhdG/fis	tRNA‑dihydrouridine synthase B/DNA‑binding protein Fis

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	3,339,158	0	A→C	100.0%	159.7	48	intergenic (+22/‑4)	yhdG/fis	tRNA‑dihydrouridine synthase B/DNA‑binding protein Fis
Reads supporting (aligned to +/- strand):  new base (24/24):  ref base (0/0):  total (24/24)

AATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGT  >  REL606/3339125‑3339190                                  |                                 aaTTTTGCGTAAACAGAAATAAAGAGCTGACAGCAc                                >  1:3424855/1‑36 (MQ=255)  aTTTTGCGTAAACAGAAATAAAGAGCTGACAGCACt                               <  1:5245139/36‑1 (MQ=255)   ttttGCGTAAACAGAAAGAAAGAGCTGACAGCACTa                              <  1:6740960/36‑1 (MQ=25)    tttGCGTAAACAGAAATAAAGAGCTGACAGCACTAt                             <  1:3733886/36‑1 (MQ=255)    tttGCGTAAACAGAAATAAAGAGCTGACAGCACTAt                             >  1:89192/1‑36 (MQ=255)    tttGCGTAAACAGAAATAAAGAGCTGACAGCACTAt                             <  1:768788/36‑1 (MQ=255)    tttGCGTAAACAGAAATAAAGAGCTGACAGCACTAt                             >  1:5102575/1‑36 (MQ=255)     ttGCGTAAACAGAAATAAAGAGCTGACAGCACTATg                            >  1:2050495/1‑36 (MQ=255)      tGCGTAAACAGAAATAAAGAGCTGACAGCACTATGt                           <  1:339432/36‑1 (MQ=255)       gCGTAAACAGAAATAAAGAGCTGACAGCACTATGtt                          <  1:6090691/36‑1 (MQ=255)       gCGTAAACAGAAATAAAGAGCTGACAGCACTATGtt                          <  1:5527238/36‑1 (MQ=255)       acGTAAACAGAAATAAAGAGCTGACAGCACTATGtt                          <  1:919511/35‑1 (MQ=255)        cGTAAACAGAAATAAAGAGCTGACAGCACTATGTTc                         <  1:5588488/36‑1 (MQ=255)        cGTAAACAGAAATAAAGAGCTGACAGCACCATGtcc                         >  1:366208/1‑34 (MQ=21)         gTAAACAGAAATAAAGAGCTGACAGCACTATGTTCg                        >  1:2447237/1‑36 (MQ=255)         gTAAACAGAAATAAAGAGCTGACAGCACTATGTTCg                        >  1:7089536/1‑36 (MQ=255)         gTAAACAGAAATAAAGAGCTGACAGCACTATGTTCg                        <  1:3873136/36‑1 (MQ=255)          tAAACAGAAATAAAGAGCTGACAGCACTATGTTCGa                       <  1:4152953/36‑1 (MQ=255)           aaaCAGAAATAAAGAGCTGACAGCACTATGTTCGaa                      <  1:2075911/36‑1 (MQ=255)           aaaCAGAAATAAAGAGCTGACAGCACTATGTTCGaa                      <  1:6985374/36‑1 (MQ=255)           aaaCAGAAATAAAGAGCTGACAGCACTATGTTCGaa                      <  1:3892080/36‑1 (MQ=255)           aaaCAGAAATAAAGAACTCACAGCACTATGTTCGaa                      <  1:466994/36‑1 (MQ=255)            aaCAGAAATAAAGAGCTGACAGCACTATGTTCGaac                     >  1:1178615/1‑36 (MQ=255)            aaCAGAAATAAAGAGCTGACAGCACTATGTTCGaac                     >  1:1701343/1‑36 (MQ=255)             aCAGAAATAAAGAGCTGACAGCACTATGTTCGaaca                    >  1:3297216/1‑36 (MQ=255)             aCAGAAATAAAGAGCTGACAGCACTATGTTCGaaca                    >  1:3114524/1‑36 (MQ=255)              cAGAAATAAAGAGCTGACAGCACTATGTTCGaacaa                   <  1:1111307/36‑1 (MQ=255)              cAGAAATAAAAAACTGACAGCACTATGTTCGaacaa                   <  1:551724/36‑1 (MQ=21)               aGAAATAAAGAGCTGACAGCACTATGTTCGaacaac                  >  1:194947/1‑36 (MQ=255)                gAAATAAAGAGCTGACAGCACTATGTTCGAACAAcg                 >  1:7295455/1‑36 (MQ=255)                  aaTAAAGAGCTGACAGCACTATGTTCGAACAAcgcg               >  1:4812678/1‑36 (MQ=255)                   aTAAAGAGCTGACAGCACTATGTTCGAACAACGCGt              <  1:256298/36‑1 (MQ=255)                   aTAAAGAGCTGACAGCACTATGTTCGAACAACGCGt              <  1:1699773/36‑1 (MQ=255)                    tAAAGAGCTGACAGCACTATGTTCGAACAACGCGTa             >  1:6405777/1‑36 (MQ=255)                     aaaGTGCTGACAGCACTATGTTCGAACAACGCGTaa            <  1:713281/36‑1 (MQ=25)                     aaaGGGCTGACAGCACTATGTTCGAACAACGCGTaa            <  1:517003/36‑1 (MQ=25)                      aaaagCTGACAGCACTATGTTCGAACAACGCGTaaa           <  1:3322286/33‑1 (MQ=25)                      aaGAGCTGACAGCACTATGTTCGAACAACGCGTaaa           >  1:6260275/1‑36 (MQ=255)                        gagCTGACAGCACTATGTTCGAACAACGCGTTAAtt         >  1:2812441/1‑36 (MQ=25)                        gagCTGACAGCACTATGTTCGAACAACGCGTAAAtt         >  1:3905250/1‑36 (MQ=255)                         agCTGACAGCACTATGTTCGAACAACGCGTCAATTc        >  1:6355849/1‑36 (MQ=25)                         agCTGACAGCACTATGTTCGAACAACGCGTAAATTc        >  1:800911/1‑36 (MQ=255)                            tGACAGCACTATGTTCGAACAACGCGTAAATTCTGa     <  1:2883435/36‑1 (MQ=255)                             gACAGCACTATGTTCGAACAACGCGTAAATTCTGAc    <  1:6614392/36‑1 (MQ=255)                             gACAGCACTATGTTCGAACAACGCGTAAATTCTGAc    >  1:5297103/1‑36 (MQ=255)                              aCAGCACTATGTTCGAACAACGCGTAAATTCTGatg   >  1:1688229/1‑34 (MQ=37)                               cAGCACTATGTTCGAACAACGCGTAAATTCTGACGt  >  1:1905883/1‑36 (MQ=255)                               cAGCACTATGTTCGAACAACGCGTAAATTCTGACGt  >  1:618605/1‑36 (MQ=255)                                  |                                 AATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGT  >  REL606/3339125‑3339190 Base quality scores:  ATCG  < 3 ≤  ATCG  < 13 ≤  ATCG  < 24 ≤  ATCG  < 30 ≤  ATCG  < 32 ≤  ATCG