Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,288,092	G→A	R57C (CGT→TGT)	arcB	hybrid sensory histidine kinase in two‑component regulatory system with ArcA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	3,288,092	0	G→A	100.0%	155.3	46	R57C (CGT→TGT)	arcB	hybrid sensory histidine kinase in two‑component regulatory system with ArcA
Reads supporting (aligned to +/- strand):  new base (32/14):  ref base (0/0):  total (32/14)

ACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCA  >  REL606/3288059‑3288124                                  |                                 aCGGCGTAATCAGCAAACCAAAGAAGATAGAACAaa                                >  1:483503/1‑36 (MQ=255) aCGGCGTAATCAGCAAACCAAAGAAGATAGAACAaa                                <  1:1138743/36‑1 (MQ=255)   ggCGTAATCAGCAAACCAAAGAAGATAGAACAAATa                              >  1:1505084/1‑36 (MQ=255)   ggCGTAATCAGCAAACCAAAGAAGATAGAACAAATa                              >  1:6002096/1‑36 (MQ=255)      gTAATCAGCAAACCAAAGAAGATAGAACAAATAACa                           >  1:2098780/1‑36 (MQ=255)         aTCAGCAAACCAAAGAAGATAGAACAAATAACATCa                        >  1:6035536/1‑36 (MQ=255)         aTCAGCAAACCAAAGAAGATAGAACAAATAACATCa                        >  1:6314485/1‑36 (MQ=255)          tCAGCAAACCAAAGAAGATAGAACAAATAACATCaa                       >  1:4410805/1‑36 (MQ=255)          tCAGCAAACCAAAGAAGATAGAACAAATAACATCaa                       >  1:3206236/1‑36 (MQ=255)          tCAGCAAACCAAAGAAGATAGAACAAATAACATCaa                       >  1:92353/1‑36 (MQ=255)           cAGCAAACCAAAGAAGATAGAACAAATAACATCAAt                      >  1:6652764/1‑36 (MQ=255)           cAGCAAACCAAAGAAGATAGAACAAATAACATCAAt                      <  1:3507936/36‑1 (MQ=255)            aGCAAACCGAAGAAGCTAGAACAAATAACATCAATg                     >  1:5992727/1‑36 (MQ=21)            aGCAAACCAAAGAAGATAGAACAAATAACATCAATg                     <  1:5088755/36‑1 (MQ=255)             gCAAACCAAAGAAGATAGAACAAATAACATCAATGc                    <  1:4584927/36‑1 (MQ=255)               aaaCCAAAGAAGATAGAACAAATAACATCAATGCtt                  <  1:2099848/36‑1 (MQ=255)                aaCCAAAGAAGATAGAACAAATAACATCAATGCttt                 >  1:6866864/1‑36 (MQ=255)                 aCCAAAGAAGATAGAACAAATAACATCAATGCTTTc                >  1:3408056/1‑36 (MQ=255)                  ccAAAGAAGATAGAACAAATAACATCAATGCTTTCg               <  1:1364436/36‑1 (MQ=255)                   cAAAGAAGATAGAACAAATAACATCAATGCTTTCGa              >  1:1900865/1‑36 (MQ=255)                   cAAAGAAGATAGAACAAATAACATCAATGCTTTCGa              >  1:6653240/1‑36 (MQ=255)                   cAAAGAAGATAGAACAAATAACATCAATGCTTTCGa              >  1:3640128/1‑36 (MQ=255)                   cAAAGAAGATAGAACAAATAACATCAATGCTATcaa              >  1:1208116/1‑34 (MQ=21)                    aaaGAAGATAGAACAAATAACATCAATGCTTTCGAc             >  1:2755693/1‑36 (MQ=255)                     aagaagATAGAACAAATAACATCAATGCTTTCGAcc            >  1:85792/1‑36 (MQ=255)                      agaagaTAGAACAAATAACATCAATGCTTTCGACCt           >  1:928301/1‑36 (MQ=255)                      agaagaTAGAACAAATAACATCAATGCTTTCGACCt           <  1:4457757/36‑1 (MQ=255)                      agaagaTAGAACAAATAACATCAATGCTTTCGACCt           >  1:358566/1‑36 (MQ=255)                      agaagaTAGAACAAATAACATCAATGCTTTCGACCt           >  1:7012416/1‑36 (MQ=255)                      agaagaTAGAACAAATAACATCAATGCTTTCGACCt           >  1:1864204/1‑36 (MQ=255)                        aagaTAGAACAAATAACATCAATGCTTTCGACCTGa         <  1:4228681/36‑1 (MQ=255)                         agaTTGAACAAATAACATCAATGCTTTCGACCTGAc        <  1:4048474/36‑1 (MQ=25)                         agaTAGAACAAATAACATCAATGCTTTCGACCTGAc        >  1:536369/1‑36 (MQ=255)                         agaTAGAACAAATAACATCAATGCTTTCGACCTGAc        >  1:6534279/1‑36 (MQ=255)                          gaTAGAACAAATAACATCAATGCTTTCGACCTGAcc       >  1:1583155/1‑36 (MQ=255)                          gaTAGAACAAATAACATCAATGCTTTCGACCTGAcc       >  1:6645481/1‑36 (MQ=255)                           aTAGAACAAATAACATCAATGCTTTCGACCTGACCa      >  1:2873623/1‑36 (MQ=255)                           aTAGAACAAATAACATCAATGCTTTCGACCTGACCa      >  1:4745090/1‑36 (MQ=255)                            tAGAACAAATAACATCAATGCTTTCGACCTGACCAt     <  1:5878860/36‑1 (MQ=255)                             aGAACAAATAACATCAATGCTTTCGACCTGACCATg    <  1:6024926/36‑1 (MQ=255)                             aGAACAAATAACATCAATGCTTTCGACCTGACCATg    >  1:5771856/1‑36 (MQ=255)                              gAACAAATAACATCAATGCTTTCGACCTGACCATgc   >  1:1607104/1‑36 (MQ=255)                              gAACAAATAACATCAATGCTTTCGACCTGACCATgc   <  1:6884015/36‑1 (MQ=255)                              gAACAAATAACATCAATGCTTTCGACCTGACCATgc   <  1:7297675/36‑1 (MQ=255)                               aaCAAATAACATCAATGCTTTCGACCTGACCATgca  >  1:6591419/1‑36 (MQ=255)                               aaCAAATAACATCAATGCTTTCGACCTGACCATgca  <  1:7464872/36‑1 (MQ=255)                                  |                                 ACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCA  >  REL606/3288059‑3288124 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 30 ≤  ATCG  < 32 ≤  ATCG